National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Candidiasis has emerged as an alarming opportunistic disease with the increase in number of patients who are immunocompromised, aged, receiving prolonged antibacterial and aggressive cancer chemotherapy or undergoing invasive surgical procedures and organ transplantation. In last few decades, there have been numerous reports of Candida infections in India. Candida albicans is considered to be the commonest and most virulent pathogenic species of the genus Candida. The clinical specimens investigated included cervical swab constituting 41(34.17%), skin scrapings 18(15%), oral swabs 14(11.67%), nail scrapings 12(10%), ear swabs 11(9.17%), sputum 10(8.33%) followed by pus 7(5.83%), urine 3(2.50%), blood 2(1.67%) and stool 2(1.67%). These samples were collected from Sharda Medical College & Hospital a tertiary care hospital. Candida species were identified from clinical samples by microscopy (KOH, Wet mount), culture on SDA and urease test. Further the isolates are speciated by germ tube test, CMA morphology, CHROM agar, sugar assimilation and sugar fermentation tests. Antifungal susceptibility was done by Disc diffusion method. Candidiasis was found to be occurring in all ages but predominant in 20-40 age group with slight increase in extreme ages. Females constituted 55% with ratio of male to female 1:1.22. The most common form of candidiasis found to be Candidial vulvovaginitis (34%) followed by intertrigo (15%). Pregnancy (21.67%) and HIV infection (20.83) appeared to be the major predisposing factor followed by diabetes (15.83%). Candida albicans (74.17%) found to be the major species isolated followed by non albicans candida (25.87%) mainly C. tropicalis (17.5%), C. glabrata (6.67%) and C. krusei (1.67%). Antifungal susceptibility testing results showed the resistance to commonly used antifungal agents in varying proportions in all types of species isolated. Resistance for Fluconazole was 15.83%, Ketoconazole 10%, Nystatin 4.17% and Amphotericin B 1.67%. Disc diffusion method is simple reliable and easy to perform and should be suitably standardized in the laboratory.
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