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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(7): 1711-1720
DOI: https://doi.org/10.20546/ijcmas.2017.607.206


In Vitro Regeneration of Parthenocarpic Cucumber (Cucumis sativus L.)
Ajay Bhardwaj*, T. Pradeepkumar and C. Varun Roch
Biotechnology Laboratory, Department of Olericulture, College of Horticulture, Kerala Agricultural University, Vellanikkara-680656, Kerala, India
*Corresponding author
Abstract:

The present investigation was carried out in Biotechnology Laboratory, Department of Olericulture, KAU, Vellanikkara with four genotypes of cucumber comprising gynoecious, monoecious and two parthenocarpic genotypes for in vitro plant regeneration. Seeds were grown in the culture bottles quarterly filled with half strength MS basal medium. Cotyledonary leaf explants were then transferred to eight media compositions of half strength MS medium with varying IAA and BAP concentrations in the culture test tubes. It was found that different media compositions exhibited varying responses with all the genotypes. The gynoecious and parthenocarpic cucumber genotypes were early to germinate than the monoecious cucumber genotype with 100 per cent germination rate. For shoot initiation, on an average, M8 media composition (Half MS + 0.5mg/l IAA + 2mg/l BAP) was found to be superior with respect to days taken for shoot initiation and response (%) whereas for root initiation, M3 (Half MS + 0.25mg/l IAA) was found to be better and for callus initiation, M5 media composition (Half MS +0.25mg/l IAA + 2mg/l BAP) was found to be the best. One can easily go for shooting, rooting and callusing phase by utilizing the above observed media compositions as an alternative for early and fast regeneration method to maintain and multiply various cucumber genetic stocks. Differential response of genotypes point towards the need for standardizing specific media for each cultivar and should be the initial step for breeding parthenocarpic, gynoecious cucumber hybrids.


Keywords: Cucumber, In vitro Regeneration, Cotyledon, Parthenocarpy, Callus culture, Leaf explant.
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How to cite this article:

Ajay Bhardwaj, T. Pradeepkumar and Varun Roch, C. 2017. In Vitro Regeneration of Parthenocarpic Cucumber (Cucumis sativus L.).Int.J.Curr.Microbiol.App.Sci. 6(7): 1711-1720. doi: https://doi.org/10.20546/ijcmas.2017.607.206