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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
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Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(7): 2527-2539
DOI: https://doi.org/10.20546/ijcmas.2017.607.298


Molecular Characterization and their Phylogenetic Relationship Based on Mitochondrial Cytochrome Oxidase I of Brinjal Shoot and Fruit Borer, Leucinodes orbonalis (Guenée) (Lepidoptera: Pyralidae)
S. Murali1*, S.K. Jalali2, A.N. Shylesha2, T.M. Shivalinga Swamy2, Reeta Baskar2 and Prathibha Mruthunjayaswamy2
1CSB, RSRS, Jammu - 181101, Jammu, India
2NBAIR, Bengaluru - 560024, Karnataka, India
*Corresponding author
Abstract:

The shoot and fruit borer, Leucinodes orbonalis has a countrywide distribution and has been categorized as the most destructive and most serious pest causing huge losses in brinjal. In the present study total of 21 populations of L. orbonalis collected mainly from Karnataka and also from Andhra Pradesh, New Delhi and Uttar Pradesh were molecularly characterized. The sequencing resulted in 600-636 bp product of CO I gene and amino acid composition of nucleotide, which varied from 195-206 in different populations. The certain amino acids were either present nor absent in different populations indicating their diversity. The pair-wise genetic distance of L. orbonalis populations ranged from 0.00 to 0.051, suggesting that there was variation in population of L. orbonalis from different locations. The highest genetic distance was observed from the population of Udupi district i.e. 0.051, followed by Koppal district is 0.047, Raichur, Chitoor, Vijayapura, Bagalkot and Guntur population having genetic distance of about 0.039 and other populations have different genetic distances which are related to each other. Therefore, it is inferred that there was no significant molecular diversity within L. orbonalis of different geographical locations of India with respect to COI but there is difference in the amino acid composition and genetic distance among the populations.


Keywords: Brinjal, L. orbonalis, Genetic distance, Population, COI.
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How to cite this article:

Murali, S., S.K. Jalali, A.N. Shylesha, T.M. Shivalinga Swamy, Reeta Baskar and Prathibha Mruthunjayaswamy. 2017. Molecular Characterization and their Phylogenetic Relationship Based on Mitochondrial Cytochrome Oxidase I of Brinjal Shoot and Fruit Borer, Leucinodes orbonalis (Guenée) (Lepidoptera: Pyralidae).Int.J.Curr.Microbiol.App.Sci. 6(7): 2527-2539. doi: https://doi.org/10.20546/ijcmas.2017.607.298