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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(7): 2476-2484
DOI: https://doi.org/10.20546/ijcmas.2017.607.292


Identification of the Suitable Hardening Protocol and Hardening Medium in Micropropagation of Gerbera (Gerbera jamesonii Bolus)
Vivek Kumar Singh1*, V.M. Prasad1, Supriya Kumari2, Preeti Rajoria3 and Pragati Misra3
1Department of Horticulture, Naini Agricultural Institute, Faculty of Agriculture, SHUATS, Allahabad 211007, India
2Department of Microbiology and Fermentation Technology,
3Tissue Culture Lab, Department of Molecular and Cellular Engineering, Jacob Institute of Biotechnology and Bioengineering, Faculty of Engineering and Technology, SHUATS, Allahabad 211007, India
*Corresponding author
Abstract:

An experiment was employed to identify the suitable rooting hormone and a potential hardening medium for gerbera micropropagation techniques. IBA (Indole Butyric Acid) and GA3 (Gibbrelic Acid) are taken for the study of rooting and Sand, Cocopeat, Vermicompost and Vermiculite selected alone or in combinations for the study of hardening in gerbera. Different parameters on root induction were taken similarly morphological and biochemical were parameters were studies in harden plants of gerbera. On the basis of different parameter it is concluded that higher rate of IBA (3 ppm and 4 ppm) lower concentration of GA3 was found good but overall performance of IBA was best. In the hardening process among the different growing medium were tested. The combined use of Sand, Cocopeat, vermicompost and vermiculite gives excellent result but alone they were failed to give desirable result.


Keywords: Gerbera, Micropropagation, Rooting, Hardening and medium.
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How to cite this article:

Vivek Kumar Singh, V.M. Prasad, Supriya Kumari, Preeti Rajoria and Pragati Misra. 2017. Identification of the Suitable Hardening Protocol and Hardening Medium in Micropropagation of Gerbera (Gerbera jamesonii Bolus).Int.J.Curr.Microbiol.App.Sci. 6(7): 2476-2484. doi: https://doi.org/10.20546/ijcmas.2017.607.292