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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(7): 2454-2461
DOI: https://doi.org/10.20546/ijcmas.2017.607.290


Identification of Selective Agents Concentrations for Optimal Plant Regeneration from Transformed Calli and Immature Embryos in Wheat
Ramandeep Kaur Jhinjer1*, Navtej Singh Bains2 and Satbir Singh Gosal1
1School of Agricultural Biotechnology and
2Department of Plant Breeding and Genetics,
Punjab Agricultural University, Ludhiana 141004 Punjab, India
*Corresponding author
Abstract:

Success rate in selection of putative transformants is mainly dependent upon the optimum concentration of selective agent. Effect of antibiotic concentrations of hygromycin (10, 20, 30 and 50 mg/L) and paromomycin (10, 20, 30 and 50 mg/L) in callusing and regeneration media on immature embryo-derived calli was evaluated using wheat variety PBW621. Hygromycin at 10 mg/L and paromomycin at 15 mg/L gave about 50% regeneration from calli (compared to more than 90% in control) leading to identification of these concentrations as optimal. In a second experiment, seedlings derived from immature embryos were subjected to hygromycin, kanamycin and paromomycin at 5, 10, 15, 20 and 25 mg/L. Hygromycin and paromomycin gave 100 percent killing of seedlings at 20 and 25 mg/L concentrations, respectively, and were identified as optimal concentrations for screening of seedlings. Kanamycin treated seedlings turned albino even at lowest concentration of 5 mg/L. A similar response had been observed in case of calli, showing relative unsuitability of kanamycin as a selective agent.


Keywords: Triticum aestivum L., Hygromycin, Paromomycin, Callus, Regeneration, Seedling.
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How to cite this article:

Ramandeep Kaur Jhinjer, Navtej Singh Bains and Satbir Singh Gosal. 2017. Identification of Selective Agents Concentrations for Optimal Plant Regeneration from Transformed Calli and Immature Embryos in WheatInt.J.Curr.Microbiol.App.Sci. 6(7): 2454-2461. doi: https://doi.org/10.20546/ijcmas.2017.607.290