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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(7): 2336-2343
DOI: https://doi.org/10.20546/ijcmas.2017.607.276


Evaluation of Protective Capacity of Glycoprotein Based DNA Vaccine against Rabies Virus
Neha R. Tomar1*, Rajiv Kumar2 and Anil Kumar3
1Department of Zoology, Kumaun University, S. S. J. Campus, Almora, Uttarakhand, India
2Division of Animal Genetics and Breeding, C. S. W. R. I., Avikanagar, Rajasthan, India
3Department of Molecular Biology and Genetic Engineering, College of Basic Sciences and Humanities, G. B. Pant University of Agriculture and Technology, Pantnagar, Uttarakhand, India
*Corresponding author
Abstract:

Rabies is a re-emerging and fatal infectious disease in Asia mainly caused by exposure to rabid dogs. In spite of great advances in virology there is yet no cure for rabies. In recent times newer modality of disease prevention like genetic vaccines and other advanced vaccines have been taken into account. Prevention of dog rabies would be the most effective way to stop rabies transmission to humans. However, vaccinating stray dogs in urban and rural areas using conventional vaccines is always difficult and is not cost-effective for use in most areas. In this study, DNA vaccine encoding the antigenic glycoprotein under the CMV promoter and Kozak sequence was developed and tried in mice by i. m. inoculation. Results showed that 87.5% (7/8) of the immunized mice had developed virus neutralizing antibodies (VNA). The antibody titer after 30 days was 4624. This vaccine afforded 87.5% protection against challenge dose 50 LD50 of CVS-24 after 30 days post challenge. This demonstrated that the DNA vaccine pcDNA-RVG could be administered and it could be effective for the vaccination of dogs to prevent the canine rabies.


Keywords: DNA vaccine, Rabies, Kozak sequence, LD50, ELISA, Antibody titre.
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How to cite this article:

Neha R. Tomar, Rajiv Kumar and Anil Kumar. 2017. Evaluation of Protective Capacity of Glycoprotein Based DNA Vaccine against Rabies Virus.Int.J.Curr.Microbiol.App.Sci. 6(7): 2336-2343. doi: https://doi.org/10.20546/ijcmas.2017.607.276