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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(7): 2263-2273
DOI: https://doi.org/10.20546/ijcmas.2017.607.266


Curcumin Enhanced Cutaneous Wound Healing by Modulating Cytokines and Transforming Growth Factor in Excision Wound Model in Rats
Raju Prasad1*, Dhirendra Kumar2, Vinay Kant2, Surendra K. Tandan2 and Dinesh Kumar2
1Department of Veterinary Pharmacology & Toxicology, Ranchi Veterinary College,Birsa Agricultural University, Kanke (Jharkhand), Pin 834 006, India
2Division of Pharmacology and Toxicology, IVRI, Izatnagar, Bareilly, UP
*Corresponding author
Abstract:

The present study was aim to evaluate the time-dependent wound healing effects of curcumin ointment (0.3%) in excision wound model in albino rats. Thirty six acclimatized rats were equally divided into two groups and ~2×2 cm2 open excision-type wound was created in rats in Group I (control) and Group II (treated). Ointment base and 0.3% w/w curcumin ointment were topically applied once daily for 14 days in group I and group II rats respectively and healing parameters were analyzed on day 3, 7, 14 post-wounding. Progress in healing was evaluated by following parameters i.e. wound contraction, supported by photographic visualization, protein levels of TNF-α, (Tumor necrosis factor- α), IL-10 (Interleukin-10), TGF-β1 (Transforming growth factor- β1) by ELISA test and immunohistochemistry of angiogenic marker protein CD-31 (Cluster of differentiation-31). The per cent (%) wound contraction was significantly (P<0.01) increased in curcumin-treated group, as compared to control group on day 7, 10 and 14. The application of curcumin markedly decreased the level of TNF- α on 3rd day onwards whereas, the levels of IL-10 were increased significantly (P<0.01) in treated group from 3rd day onwards. The levels of growth factors TGF-β1 were also highly up-regulated on day 3, 7 and 14. On the day 14, section of curcumin treated group had well formed capillary vessels lined by CD-31 positive endothelial cells whereas, in control, ill formed capillary vessel scattered in immature granulation tissue were found. In conclusion, the curcumin ointment has promising wound healing potential in a time-dependent manner on topical application.


Keywords: Curcumin, Wound Healing, IL-10, TNF-α, TGF-β1, CD-31.
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How to cite this article:

Raju Prasad, Dhirendra Kumar, Vinay Kant, Surendra K. Tandan and Dinesh Kumar. 2017. Curcumin Enhanced Cutaneous Wound Healing by Modulating Cytokines and Transforming Growth Factor in Excision Wound Model in Rats.Int.J.Curr.Microbiol.App.Sci. 6(7): 2263-2273. doi: https://doi.org/10.20546/ijcmas.2017.607.266