National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The present study was aim to evaluate the time-dependent wound healing effects of curcumin ointment (0.3%) in excision wound model in albino rats. Thirty six acclimatized rats were equally divided into two groups and ~2×2 cm2 open excision-type wound was created in rats in Group I (control) and Group II (treated). Ointment base and 0.3% w/w curcumin ointment were topically applied once daily for 14 days in group I and group II rats respectively and healing parameters were analyzed on day 3, 7, 14 post-wounding. Progress in healing was evaluated by following parameters i.e. wound contraction, supported by photographic visualization, protein levels of TNF-α, (Tumor necrosis factor- α), IL-10 (Interleukin-10), TGF-β1 (Transforming growth factor- β1) by ELISA test and immunohistochemistry of angiogenic marker protein CD-31 (Cluster of differentiation-31). The per cent (%) wound contraction was significantly (P<0.01) increased in curcumin-treated group, as compared to control group on day 7, 10 and 14. The application of curcumin markedly decreased the level of TNF- α on 3rd day onwards whereas, the levels of IL-10 were increased significantly (P<0.01) in treated group from 3rd day onwards. The levels of growth factors TGF-β1 were also highly up-regulated on day 3, 7 and 14. On the day 14, section of curcumin treated group had well formed capillary vessels lined by CD-31 positive endothelial cells whereas, in control, ill formed capillary vessel scattered in immature granulation tissue were found. In conclusion, the curcumin ointment has promising wound healing potential in a time-dependent manner on topical application.
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