National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The omega fatty acids are a group of compounds that are included under essential fatty acids. Alpha-linolenic acid is an essential omega-3 fatty acid, needed for normal human growth and development. The silkworm pupae oil contains 25.7% of alpha linolenic acid. There are many methods adopted for purification and quantification of ALA. However, HPLC technique was adopted as an established method of quantifying ALA from vegetable oils. This method has been modified to quantify ALA from silkworm pupal residue oil. Alpha linolenic acid was isolated by using two step crystallization methods. Silkworm pupal oil contains both unsaturated and polyunsaturated fatty acids. Unsaturated fatty acids are removed in first step of crystallization at 4ºC for about 24 hours. Polyunsaturated fatty acids were crystallized at -18ºC. Isolated alpha linolenic acid was analyzed by HPLC against sigma Aldrich standard. On the basis of UV/VIS spectra of fatty acids, the poly unsaturated fatty acids have absorption peaks about 210nm to 300nm.The polyunsaturated fatty acids (ALA) absorption peaks were observed at 210 nm. Monitoring of ALA was carried out at 210 nm. The ALA in the sample was identified by comparing the retention time with that of respective standard. The retention time of ALA and sample was 4.39. Quantification was carried out by integration of the peaks using external standard.
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