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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(7): 2202-2206
DOI: https://doi.org/10.20546/ijcmas.2017.607.258


Isolation and Detection of Alpha Linolenic Acid from Silkworm Pupal Residue Oil (Bombyx mori L.) Using HPLC
B.S. Vidhathri1, V.R. Ramakrishna Parama2, C.T. Subbarayappa2, T.K. Narayanaswamy1, R. Muthuraju3, D.S. Mahesh1* and D.N. Vidyashree3
1Department of Sericulture, UAS, GKVK, Karnataka, India
2Department of Soil Science and Agricultural Chemistry, UAS, GKVK, Karnataka, India
3Department of Agricultural Microbiology, UAS, GKVK, Karnataka, India
*Corresponding author
Abstract:

The omega fatty acids are a group of compounds that are included under essential fatty acids. Alpha-linolenic acid is an essential omega-3 fatty acid, needed for normal human growth and development. The silkworm pupae oil contains 25.7% of alpha linolenic acid. There are many methods adopted for purification and quantification of ALA. However, HPLC technique was adopted as an established method of quantifying ALA from vegetable oils. This method has been modified to quantify ALA from silkworm pupal residue oil. Alpha linolenic acid was isolated by using two step crystallization methods. Silkworm pupal oil contains both unsaturated and polyunsaturated fatty acids. Unsaturated fatty acids are removed in first step of crystallization at 4ºC for about 24 hours. Polyunsaturated fatty acids were crystallized at -18ºC. Isolated alpha linolenic acid was analyzed by HPLC against sigma Aldrich standard. On the basis of UV/VIS spectra of fatty acids, the poly unsaturated fatty acids have absorption peaks about 210nm to 300nm.The polyunsaturated fatty acids (ALA) absorption peaks were observed at 210 nm. Monitoring of ALA was carried out at 210 nm. The ALA in the sample was identified by comparing the retention time with that of respective standard. The retention time of ALA and sample was 4.39. Quantification was carried out by integration of the peaks using external standard.


Keywords: Alpha-linolenic acid, Silkworm pupal residue, Extraction, Isolation.
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How to cite this article:

Vidhathri, B.S., V.R. Ramakrishna Parama, C.T. Subbarayappa, T.K. Narayanaswamy, R. Muthuraju, D.S. Mahesh and Vidyashree, D.N. 2017. Isolation and Detection of Alpha Linolenic Acid from Silkworm Pupal Residue Oil (Bombyx mori L.) using HPLC.Int.J.Curr.Microbiol.App.Sci. 6(7): 2202-2206. doi: https://doi.org/10.20546/ijcmas.2017.607.258