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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(7): 2155-2166
DOI: https://doi.org/10.20546/ijcmas.2017.607.253


Effect of Ammonia Concentration on the Nitrification Potential of Ammonia Oxidizing Bacterial Isolates from Fish Processing Waste Effluents
A. Devivaraprasad Reddy1, G. Subrahmanyam3, M.M. Dechamma2, B.B. Nayak4, Iddya Karunasagar5 and Indrani Karunasagar6*
1Krishi Vigyan Kendra, Dr. YSR Horticultural University, Venkataramannagudem, West Godavari District, Andhra Pradesh – 534 101, India
2Department of Fisheries Microbiology, College of Fisheries, Karnataka Veterinary, Animal and Fisheries Sciences University, Mangalore-575 002, India
3Central Muga Eri Research and Training Institute, Central Silk Board,Lahdogiarh-785700, Jorhat, Assam, India
4Fish Harvest and Post Harvest Division, Central Institute of Fisheries Education,Mumbai, Maharastra, India
5Senior Fishery Industry Officer, FAO, Rome
6Department of Biomedical Science, Nitte University Center for Science Education and Research, UNESCO MIRCEN for Biotechnology, K.S. Hegde Medical Academy, Nitte University, Mangalore – 575 018, India
*Corresponding author
Abstract:

The present investigation was carried out to understand the effect of ammonia concentrations on the rate of ammonia-oxidizing activity of ammonia oxidizing bacteria (AOB) isolates. Maximum ammonium removal by all the isolates was found at 3 ppm ammonium concentration. However, AOB-12 and Nitrosomonas europaea ATCC 19718 cultures were found to be efficient at low ammonium concentration (3 ppm and 8 ppm), whereas AOB-21and AOB consortia (AOB-12+AOB-21) performed well at high ammonium concentrations (25, 50, 100 and 200 ppm). These results were corroborated with ammonia monooxyginase (amoA) gene copy numbers estimated by quantitative real time PCR (q-PCR). The maximum amoA gene copy numbers were recorded in N. europaea ATCC 19718 at ammonia level of 50 ppm followed by AOB-12 at 25 ppm and the consortia having maximum copy numbers at 100 ppm. This study indicates the promise of using native AOB cultures to remove ammonia from the waste waters prior to release into the environment.


Keywords: Ammonia oxidation, AOB strains, Ammonia concentrations, amoA gene, qPCR.
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How to cite this article:

Devivaraprasad Reddy, A., G. Subrahmanyam, M.M. Dechamma, B.B. Nayak, Iddya Karunasagar and Indrani Karunasagar. 2017. Effect of Ammonia Concentration on the Nitrification Potential of Ammonia Oxidizing Bacterial Isolates from Fish Processing Waste Effluents.Int.J.Curr.Microbiol.App.Sci. 6(7): 2155-2166. doi: https://doi.org/10.20546/ijcmas.2017.607.253