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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(7): 1618-1624
DOI: https://doi.org/10.20546/ijcmas.2017.607.195


Genetic Diversity of Trichoderma sp. from Rhizosphere Regions of Different Cropping Systems using RAPD Markers
A. Ranga Rani1*, S. Khayum Ahammed2 and A.K. Patibanda3
1Department of Plant Pathology, S.V. Agricultural College, Tirupati, India
2Scientist, Regional Agricultural Research Station, Nandyal, ANGRAU, India
3Department of plant pathology, Agricultural College, Bapatla, India
*Corresponding author
Abstract:

A total of nine Trichoderma isolates were obtained from 27 rhizosphere samples collected from different cropping systems i.e. groundnut, redgram and tomato. A random amplified polymorphic DNA (RAPD) marker was used to estimate the genetic variation among 9 isolates of Trichoderma. These isolates were characterized using 15 random primers of the OPA and OPM series. Out of which 9 primers gave reproducible and scorable band with high percentage of polymorphism. Fifteen selected primers gave total of 207 amplification products, out of which 196 were polymorphic. The maximum polymorphism (100%) was observed in PCR reaction with OPA-01, OPA-03, OPA-05, OPA-09, OPA-10, OPM-04 and OPM-20 with size ranging from 250bp to 2500 bp. The genetic distance between each isolate was calculated, and cluster analysis was used to generate a dendrogram showing the relationship among them. The Trichoderma isolates clustered into two major groups, first group having GRT-4, GRT-5, GRT-3 and TRT-1 in one cluster and remaining isolates GRT-1, GRT-2, RRT-2, TRT-2 and RRT-1 in other cluster which in turn grouped into two sub-clusters separating isolates GRT-1, GRT-2 formed one group and RRT-2, TRT-2 and RRT-1 formed another group. Similarity matrix thus produced indicated that maximum genetic variation observed between isolates of GRT-4 and RRT-2 (90.7%) closely followed by GRT-3 with RRT-2 and TRT-2 (90.1%). Isolates GRT-4 and GRT-5 were genetically closer than any isolate with 73.8% similarity. The isolate GRT-2 was closely related to isolate TRT-2 with 31% similarity and distinctly related to TRT-1 with 16.7% similarity.


Keywords: Trichoderma, Genetic diversity, RAPD, Rhizosphere, Different cropping systems.
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How to cite this article:

Ranga Rani, A., S. Khayum Ahammed, Patibanda, A.K. 2017. Genetic Diversity of Trichoderma Sp. from Rhizosphere Regions of Different Cropping Systems using RAPD MarkersInt.J.Curr.Microbiol.App.Sci. 6(7): 1618-1624. doi: https://doi.org/10.20546/ijcmas.2017.607.195