National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
A total of nine Trichoderma isolates were obtained from 27 rhizosphere samples collected from different cropping systems i.e. groundnut, redgram and tomato. A random amplified polymorphic DNA (RAPD) marker was used to estimate the genetic variation among 9 isolates of Trichoderma. These isolates were characterized using 15 random primers of the OPA and OPM series. Out of which 9 primers gave reproducible and scorable band with high percentage of polymorphism. Fifteen selected primers gave total of 207 amplification products, out of which 196 were polymorphic. The maximum polymorphism (100%) was observed in PCR reaction with OPA-01, OPA-03, OPA-05, OPA-09, OPA-10, OPM-04 and OPM-20 with size ranging from 250bp to 2500 bp. The genetic distance between each isolate was calculated, and cluster analysis was used to generate a dendrogram showing the relationship among them. The Trichoderma isolates clustered into two major groups, first group having GRT-4, GRT-5, GRT-3 and TRT-1 in one cluster and remaining isolates GRT-1, GRT-2, RRT-2, TRT-2 and RRT-1 in other cluster which in turn grouped into two sub-clusters separating isolates GRT-1, GRT-2 formed one group and RRT-2, TRT-2 and RRT-1 formed another group. Similarity matrix thus produced indicated that maximum genetic variation observed between isolates of GRT-4 and RRT-2 (90.7%) closely followed by GRT-3 with RRT-2 and TRT-2 (90.1%). Isolates GRT-4 and GRT-5 were genetically closer than any isolate with 73.8% similarity. The isolate GRT-2 was closely related to isolate TRT-2 with 31% similarity and distinctly related to TRT-1 with 16.7% similarity.
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