National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
In present era, breeding in mango crop are still done through conventional methods which is a serious drawback as it is laborious and time consuming. Mango malformation is a serious disease causing great losses to mango industry. It is difficult to control mango malformation disease through use of any chemicals. Brassinosteroids (BR) are known to have a wide range of functions to biotic and abiotic stresses. No reports are available relating BR level with mango malformation till date. In the present study we analyzed the brassinosteroid level in malformed and healthy buds at different growth stages through transcriptomic approach by using illumina pair-end sequencing. In the present study we observed the transcripts related to BR’s, target gene of BR’s, biosynthesis, signaling pathways and differentially expressed genes (DEGs). The results indicated that biosynthesis genes were more in malformed tissues (MB-1:5, MB-2:6 & MB-3:7) compared to healthy tissues (HB-1: 5, & HB-2: 5) whereas BR signaling genes were more in healthy tissues (HB-1:24, HB-2: 26) compared to malformed tissues (MB-1:21, MB-2:20 & MB-3: 21). The brassinosteroids signaling pathway genes in healthy tissues increased from bud to panicle formation stages. Differential Expression analysis indicated that BZR-1 a signaling gene was significantly up regulated in healthy panicle formation stage compared to all malformed bud developmental stages. Among the target genes of BR’s signaling PIF, MYB-30 TF were higher in number in malformed buds compared to healthy buds at different growth stages. The results could serve as breeding targets for mango crop improvement.
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