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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(7): 1462-1475
DOI: https://doi.org/10.20546/ijcmas.2017.607.175


Transcriptomic Analysis of Brassinosteroid Biosynthesis and Signaling Pathway Genes in Healthy and Malformed Tissues of Mango Variety Amrapalli
Ashok Yadav and Usha Kalidindi*
Division of Fruits and Horticultural Technology, Indian Agricultural Research Institute, New Delhi-110012, India
*Corresponding author
Abstract:

In present era, breeding in mango crop are still done through conventional methods which is a serious drawback as it is laborious and time consuming. Mango malformation is a serious disease causing great losses to mango industry. It is difficult to control mango malformation disease through use of any chemicals. Brassinosteroids (BR) are known to have a wide range of functions to biotic and abiotic stresses. No reports are available relating BR level with mango malformation till date. In the present study we analyzed the brassinosteroid level in malformed and healthy buds at different growth stages through transcriptomic approach by using illumina pair-end sequencing. In the present study we observed the transcripts related to BR’s, target gene of BR’s, biosynthesis, signaling pathways and differentially expressed genes (DEGs). The results indicated that biosynthesis genes were more in malformed tissues (MB-1:5, MB-2:6 & MB-3:7) compared to healthy tissues (HB-1: 5, & HB-2: 5) whereas BR signaling genes were more in healthy tissues (HB-1:24, HB-2: 26) compared to malformed tissues (MB-1:21, MB-2:20 & MB-3: 21). The brassinosteroids signaling pathway genes in healthy tissues increased from bud to panicle formation stages. Differential Expression analysis indicated that BZR-1 a signaling gene was significantly up regulated in healthy panicle formation stage compared to all malformed bud developmental stages. Among the target genes of BR’s signaling PIF, MYB-30 TF were higher in number in malformed buds compared to healthy buds at different growth stages. The results could serve as breeding targets for mango crop improvement.


Keywords: Mango, Mango malformation, Brassinosteroid, Biosynthesis, Signaling, Pathways.
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How to cite this article:

Ashok Yadav and Usha Kalidindi. 2017. Transcriptomic Analysis of Brassinosteroid Biosynthesis and Signaling Pathway Genes in Healthy and Malformed Tissues of Mango Variety AmrapalliInt.J.Curr.Microbiol.App.Sci. 6(7): 1462-1475. doi: https://doi.org/10.20546/ijcmas.2017.607.175