National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The aim of the current study is the study of different parameters affecting the process of adherence of different bacterial species known to be involved in eye infection due to the use of contact lenses. Ninety patients with clinical evidence of microbial keratitis who attended the outpatient clinic corneal unit department of the Research Institute of Ophthalmology, Giza, Egypt were investigated in our study. The biofilm formation was determined using the biochemical methyl tetrazolium bromide (MTT)[(3-(4, 5-dimethyl thiazole-2-yl)-2, 5-diphenyl tetrazolium bromide]. Different factors influencing bacterial adhesion were studied. Thirty Pseudomonas aeruginosa isolates and fifteen Staphylococcus aureus isolates exhibited adherence capability to contact lenses. Pseudomonas aeruginosa adhered at higher number compared to Staphylococcus aureus. Inoculum size was the greatest influencing factor for P. aeruginosa adhesion, followed by incubation period and assay media.
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