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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(4): 175-185
DOI: http://dx.doi.org/10.20546/ijcmas.2016.504.022


Evaluation of Microbial Adhesion to Contact Lenses
Rania Abdelmonem Khattab1*, Alaa El-Din Shawky Hosny1 and Nahla Mostafa Amer2
1Microbiology and Immunology Department, Faculty of Pharmacy,Cairo University, Kasr Al-Aini 11562, Cairo, Egypt
2Police Authority Hospital, Giza, Egypt
*Corresponding author
Abstract:

The aim of the current study is the study of different parameters affecting the process of adherence of different bacterial species known to be involved in eye infection due to the use of contact lenses. Ninety patients with clinical evidence of microbial keratitis who attended the outpatient clinic corneal unit department of the Research Institute of Ophthalmology, Giza, Egypt were investigated in our study. The biofilm formation was determined using the biochemical methyl tetrazolium bromide (MTT)[(3-(4, 5-dimethyl thiazole-2-yl)-2, 5-diphenyl tetrazolium bromide]. Different factors influencing bacterial adhesion were studied. Thirty Pseudomonas aeruginosa isolates and fifteen Staphylococcus aureus isolates exhibited adherence capability to contact lenses. Pseudomonas aeruginosa adhered at higher number compared to Staphylococcus aureus. Inoculum size was the greatest influencing factor for P. aeruginosa adhesion, followed by incubation period and assay media.


Keywords: Contact Lenses,adherence,MTT.
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How to cite this article:

Rania Abdelmonem Khattab, Alaa El-Din Shawky Hosny and Nahla Mostafa Amer. 2016. Evaluation of Microbial Adhesion to Contact Lenses.Int.J.Curr.Microbiol.App.Sci. 5(4): 175-185. doi: http://dx.doi.org/10.20546/ijcmas.2016.504.022