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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(7): 1221-1228
DOI: https://doi.org/10.20546/ijcmas.2017.607.147


Mixed-Infection of Papaya Ring Spot Virus and Tomato Leaf Curl New Delhi Virus in Coccinia grandis in India
Siriya Sultana, Buddhadeb Roy and Ang Rinzing Sherpa*
Department of Botany, West Bengal State University, Berunanpukuria, Malikapur, North-24 Parganas, Barasat, Kolkata-700126, West Bengal, India
*Corresponding author
Abstract:

During survey from March 2014 to October 2015, in Barasat, West Bengal, India, incidence of symptoms suggestive of virus infection was observed in Coccinia grandis. Infected plants were showing typical symptoms including leaf curling, crinkled leaves, leaf yellowing and stunted growth. Symptomatic plant samples were tested for the presence of potyvirus and geminivirus. The occurrence of potyviruses was confirmed by ACP-ELISA, RT-PCR and nucleotide analysis of the part of genome. On the other hand, occurrences of geminiviruses were confirmed by southern blot analysis, polymerase chain reaction (PCR) and nucleotide analysis of the part of genome. Sequence analysis showed that the potyvirus from C. grandis shares 92% identity with Papaya ring spot virus (PRSV) isolate at the nucleotide level. The Geminivirus from the C. grandis shares 98 % identity with Tomato leaf curl New Delhi virus at the nucleotide level. This is the first evidence of mixed infection of Papaya ring spot virus and Tomato leaf curl New Delhi virus in C. grandis in India.


Keywords: Coccinia grandis, PRSV, ToLCNDV,ACP-ELISA,RT-PCR
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How to cite this article:

Siriya Sultana, Buddhadeb Roy and Ang Rinzing Sherpa. 2017. Mixed-Infection of Papaya Ring Spot Virus and Tomato Leaf Curl New Delhi Virus in Coccinia grandis in India.Int.J.Curr.Microbiol.App.Sci. 6(7): 1221-1228. doi: https://doi.org/10.20546/ijcmas.2017.607.147