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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(4): 127-132
DOI: http://dx.doi.org/10.20546/ijcmas.2016.504.017


Performance of Modified Hodge Test for Detection of  Carbapenemase Producing Clinical Isolates of Pseudomonas aeruginosa
S. Jayalakshmi* and Sathya Pandurangan
Department of Microbiology, Shri Sathya Sai Medical College and Research Institute, Sri Balaji Vidyapeeth University, Thiruporur, Kancheepuram (District), Tamilnadu, India
*Corresponding author
Abstract:

Pseudomonas aeruginosa is a clinically troublesome gram negative pathogen that causes a wide range of opportunistic infections and nosocomial outbreaks. Carbapenems are becoming ineffective mostly due to the emergence of carbapenemase. The aim of this study was to performance of the phenotypic methods such as Modified Hodge test (MHT) for detection of carbapenemase producing clinical isolates of Pseudomonas aeruginosa. This study was conducted in the Department of Microbiology, SSSMC&RI, which caters to 750 beds. A total of 152 Pseudomonas aeruginosa isolates from different clinical samples were taken, during the period of August 2015 to January 2016. These isolates were subjected to Modified Hodge Test. Out of 152 Pseudomonas aeruginosa isolates were tested for carbapenemase production by Modified Hodge test (MHT). Among the 152 P. aeruginosa isolates, only 50(33%) isolates were Modified Hodge test Positive and 102(67%) isolates were negative by MHT.  Modified Hodge test is a very sensitive and reliable test which can be performed in the routine laboratory test for detection of carbapenmase.


Keywords: Pseudomonas aeruginosa, Carbapenemase, Modified Hodge test (MHT), Beta-lactamase.
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How to cite this article:

Jayalakshmi, S., and Sathya Pandurangan. 2016. Performance of Modified Hodge Test for Detection of  Carbapenemase Producing Clinical Isolates of Pseudomonas aeruginosa.Int.J.Curr.Microbiol.App.Sci. 5(4): 127-132. doi: http://dx.doi.org/10.20546/ijcmas.2016.504.017