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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(7): 1002-1009
DOI: https://doi.org/10.20546/ijcmas.2017.607.121


Effect of Phytohormones and Rhizobacteria on Epithelantha micromeris Callus Growth
Daniel Corona-Álvarez1, Leonor Angélica Guerrero-Zúñiga2 and Angélica Rodríguez-Dorantes1*
1Laboratorio de Fisiología Vegetal, Departamento de Botánica, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, México City 11340, México
2Instituto Mexicano Del Petróleo, México City 07730, México
*Corresponding author
Abstract:

The employ of in vitro systems allows the dissection of complex interaction between plants and microorganisms, by the analysis of stress factors involved in the plant’s response. Sterile cell cultures are reproducible during long periods and help to the measurement of physiological activities. Plant growth promoting rhizobacteria (PGPR) are a group of microorganisms that are involved in plants’ growth promotion. This study evaluated the effect of particular phytohormones and a plant growth promoting rhizobacteria on the growth and viability of Epithelantha micromeris callus culture. E. micromeris callus growth without the inoculant, diminished in Murashige-Skoog (MS) + naphthalene acetic acid (NAA) medium (34.78%) and MS + indole acetic acid (IAA) (26.08%) experiments. In inoculated callus (B) there was a little promoting effect in: MS+NAA+B (17.39%), MS-VITAMINS+B (30.43%); MS+B (47.82%) experiments, and particularly case was MS+IAA+B experiment, where callus gain 30.43% biomass above the control values obtained. In this work, the presence of some particular auxins and their concentration on non-inoculated and inoculated Epithelantha micromeris callus with a plant growth promoting rhizobacteria, showed a diminished response and even there wasn’t a notably cell dead and the effect of the suggested bioauxins were not evident, the gain of callus fresh biomass was present.


Keywords: Indole acetic acid, Naphthalene acetic acid, Callus culture, PGPR
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How to cite this article:

Daniel Corona-Álvarez, Leonor Angélica Guerrero-Zúñiga and Angélica Rodríguez-Dorantes. 2017. Effect of Phytohormones and Rhizobacteria on Epithelantha micromeris Callus Growth.Int.J.Curr.Microbiol.App.Sci. 6(7): 1002-1009. doi: https://doi.org/10.20546/ijcmas.2017.607.121