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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(7): 857-865
DOI: https://doi.org/10.20546/ijcmas.2017.607.105


Effect of Methyl Jasmonate on Disease Severity and Expression of Plant Defensin Gene during Alternaria brassicae Infection in Arabidopsis
Snigdha Tiwari*, Dinesh Pandey, Manu Gaur and Anil Kumar
Department of Molecular Biology and Genetic Engineering, G. B. Pant University of Agriculture and Technology, Pantnagar-263145, Uttarakhand, India
*Corresponding author
Abstract:

Amongst the oilseed crops, rapeseed and mustard (Brassica spp.) play a central role in agricultural economy of the world. These crops are affected by a number of diseases which limit productivity of the crop over a wide area and one of these is Alternaria blight disease. Causal agent Alternaria brassicae, hemibiotrophic in nature and shows more propensity towards necrotrophy. Jasmonic acid (JA) acts as a defense signal against this necrotrophic pathogen by triggering Induced Systemic Resistance and induces defensin genes (PDF1.2) expression in A. thaliana leaves challenged with fungal pathogens. A study on effect of jasmonate on disease progression as well as induction of defensin gene (PDF1.2) was carry out to explore the effectiveness of Jasmonic acid in triggering defence within the host tissues. The disease severity and expression of PDF1.2 gene was examined after treatment containing Jasmonic acid and pathogen to two ecotypes of Arabidopsis at different time intervals. Methyl jasmonate treatment was found to be effective in decreasing the disease incidence by lowering the disease index in both susceptible (WS) and tolerant ecotype (Col). Transcripts of PDF1.2 accumulated at a greater level upon challenge inoculation with A. brassicae along with Jasmonic acid compared to treatment containing pathogen alone as well as Jasmonic acid alone. These results suggest the involvement of JA signaling pathways conferring resistance to A. brassicae.


Keywords: Jasmonic acid, Alternaria blight, Plant Defensin gene (PDF1.2), Brassica juncea, Arabidopsis.
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How to cite this article:

Snigdha Tiwari, Dinesh Pandey, Manu Gaur and Anil Kumar. 2017. Effect of Methyl Jasmonate on Disease Severity and Expression of Plant Defensin Gene during Alternaria Brassicae Infection in Arabidopsis.Int.J.Curr.Microbiol.App.Sci. 6(7): 857-865. doi: https://doi.org/10.20546/ijcmas.2017.607.105