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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(7): 770-778
DOI: https://doi.org/10.20546/ijcmas.2017.607.096


Detection of Methicillin Resistant, ESBL and Amp C Producing Uropathogens from a Tertiary Care Hospital in North India
Shetty Jeevan and Afroz Zarrin
Shetty Jeevan* and Afroz Zarrin
*Corresponding author
Abstract:

Uropathogens are responsible for causing one of the commonest infectious diseases in clinical practice. Empirical treatment of this infection is dependent on antibiotic sensitivity pattern of common isolates prevalent in the region. The aim of the study was to determine resistant pattern of urinary isolates and occurrence of extended spectrum beta- lactamase (ESBL) and Amp C production in them. Patients clinically suspected to be having urinary tract infection (UTI) were included in the study. This study was conducted in the Department of Microbiology, G. S Medical College & Hospital, Pilkhuwa, Uttar Pradesh over a period of 9 months from August 2016 to April 2017. The isolates were identified by conventional microbiological methods and antimicrobial susceptibility testing was done by Kirby- Bauer disk diffusion method. Detection of Extended Spectrum Beta-Lactamase (ESBL) production in Gram negative organisms was done by phenotypic confirmatory disc diffusion test (PCDT) and Methicillin resistance in Staphylococcus was carried out according to Clinical Laboratory Standards Institute (CLSI) guidelines. Amp C beta- lactamase detection was done by Amp C Disc test. Out of the 210 (16.9%) uropathogens obtained from 1236 urine samples, 166 (13.4%) were gram negative bacilli and 44(3.5 %) were gram positive cocci. Among Staphylococcus aureus (S. aureus), 24 isolates were methicillin sensitive Staphylococcus aureus (MSSA) and 12 methicillin resistant Staphylococcus aureus (MRSA). Among 166 gram negative bacilli, Escherichia coli (E. coli) was the commonest isolate (57.8%) followed by Klebsiella species (32.5%). UTI was more common in females (74.3 %) compared to male (25.7%) population. Age group of 20-30 years showed higher incidence and females predominated males in all age groups except among ≥ 60 years. Among gram negative bacilli, Imipenem showed 100% sensitivity while in gram positive cocci all isolates were sensitive to Vancomycin. ESBL production was observed in 65(39.1%) and Amp C production in 41 (24.6%) isolates of gram negative bacilli. E. coli (30.1%) was the predominant ESBL producer while plasmid mediated Amp C beta-lactamase was observed mainly in Klebsiella species (19.3%) both of which are statistically significant (p<0.05). This study reveals high prevalence of MRSA, ESBL and Amp C producing urinary pathogens in this region. It is necessary for both clinicians and microbiologists to recognize this high rate of multi drug resistance, so that effective measures may be adopted to control their spread.


Keywords: Detection, Uropathogens, Tertiary care and North India.
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How to cite this article:

Shetty Jeevan and Afroz Zarrin. 2017. Detection of Methicillin Resistant, ESBL and Amp C Producing Uropathogens from a Tertiary Care Hospital in North India.Int.J.Curr.Microbiol.App.Sci. 6(7): 770-778. doi: https://doi.org/10.20546/ijcmas.2017.607.096