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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(7): 753-759
DOI: https://doi.org/10.20546/ijcmas.2017.607.094


Evaluate the Shelf Life of Rhizobium Carrier Based Biofertilizer Stored at Different Temperatures at Different Intervals
G. Thirumal*, R. Subhash Reddy, S. Triveni, K. Damodarachari and K. Bhavya
Department of Agricultural Microbiology and Bioenergy, College of Agriculture, Professor Jaya Shankar Telangana State Agricultural University, Rajendranagar, Hyderabad - 500 030, Telangana, India
*Corresponding author
Abstract:

In the present study a total of ten Rhizobial bacterial isolates collected from different sources. These cultures were studied morphologically and biochemically for purity confirmation. The screening test results revealed that, among all Rhizobial isolates, GNR-1 showed best plant growth promoting abilities in in-vitro conditions. This promising culture was selected for main experiment toevaluate the shelf life of carrier based bio inoculants in different carrier materials stored at different temperatures in different intervals. The selected efficient PGP Rhizobium (GNR-1) and isolate was multiplied in large quantities in appropriate culture broth by incubating at 28±2°C in an incubator shaker at 120 rpm till they attained log phase with a cell load of l×l09cfu ml-1. For biofertilizer production Vermicompost, Vermiculate, Lignite, and Sodium Alginate carriers were used for bio inoculant preparation and stored at different temperatures i.e. 4ºC and 28ºC. Though the bacterial population decreased from 1st month to 8th month the survival rate of rhizobial cells was more upto 8th month at 40C. Similarly with respect to evaluation of shelf life of bacterial cells in different carrier materials like sodium alginate vermicompost lignite and vermiculite, sodium alginate based inoculants maintained good population count upto 8th month.


Keywords: Rhizobium, Pseudomonas, Carriers, Sterilization, Different Temperatures Sodium alginate.
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How to cite this article:

Thirumal, G., R. Subhash Reddy, S. Triveni, K. Damodarachari and Bhavya, K. 2017. Evaluate the Shelf Life of Rhizobium Carrier Based Biofertilizer Stored at Different Temperatures at Different IntervalsInt.J.Curr.Microbiol.App.Sci. 6(7): 753-759. doi: https://doi.org/10.20546/ijcmas.2017.607.094