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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(7): 664-672
DOI: https://doi.org/10.20546/ijcmas.2017.607.081


Isolation of Pigmented Yeasts, Extraction of Pigment and Study of Antimicrobial Property of its Pigment
Diksha R. Karanjgaokar* and Kishori S. Tarfe
Department of Biotechnology, Smt. Chandibai Himmathmal Mansukhani College, Ulhasnagar, Mumbai, Maharashtra, India
*Corresponding author
Abstract:

In nature, there exist a wide variety of microbes that can be of any economical importance to humans. A specialized group of microorganisms includes pigmented yeasts. They are yeasts that develop colored pigment as their secondary metabolites. In this study, pigmented yeasts were isolated from different sample (soil, leaves, fruits, coconut, and air) on Sabouraud’s agar. Four strains were selected that showed pigmentation. Various morphological and biochemical tests were performed for identification of the strains. The pigments were extracted in acetone and were partitioned in petroleum ether in separating funnel. The characterization of the extracted pigments was done using UV-Visible spectrophotometer. Also the antimicrobial effect of the extracted pigments was studied against E. coli, S. aureus, K. pneumoniae and C. diphtheriae. The pigments extracted from one of the isolate showed increase in antimicrobial property against K. pneumoniae with increase in volume of pigment.


Keywords: Pigmented yeast, Isolation, Partitioning, UV-Visible spectrophotometer, Antimicrobial study.
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How to cite this article:

Diksha R. Karanjgaokar, Kishori S. Tarfe. 2017. Isolation of Pigmented Yeasts, Extraction of Pigment and Study of Antimicrobial Property of its PigmentInt.J.Curr.Microbiol.App.Sci. 6(7): 664-672. doi: https://doi.org/10.20546/ijcmas.2017.607.081