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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(7): 656-663
DOI: https://doi.org/10.20546/ijcmas.2017.607.080


Analysis of Genetic Diversity of 12 Genotypes of Glycine max by Using RAPD Marker
Shashank A. Tidke1, D. Ramakrishna1, S. Kiran1, Georgina Kosturkova2, G.A. Ravishankar1
1Dr. C.D. Sagar Center for Life Sciences, Dayananda Sagar College of Engineering (Visvesvaraya Technological University), Kumaraswamy Layout, Bangalore 560078, India
2Bulgarian Academy of Sciences, Sofia, Bulgaria
*Corresponding author
Abstract:

Soybean (Glycine max) is an essential legume crop in developing nations where it serves to meet the increasing demands for protein, edible oil and calories. In this study, we analysed genetic diversity of 12 soybean genotypes using Random Amplified Polymorphic DNA (RAPD) molecular markers. A total of 10 RAPD primers were used for the screening of 12germplasm line out of which 525 fragments were amplified with 10 random primers and 74.85 % were polymorphic. Genetic similarity matrix based on Jaccard Similarity Coefficients of soybean genotypes ranged from 0.30 to 0.77. These coefficients were used to construct a dendrogram using the unweighted pair group of arithmetic means (UPGMA). All 12 soybean genotypes were grouped into six clusters. The largest cluster consisted of 5 genotypes. The highest similarity among the soybean varieties were observed between JS-02-21 and DSB-61 (0.756). Present study indicated a great deal of germplasm diversity among these 12 genotypes. Our study revealed that RAPD technology is potentially simple, rapid, reliable and an effective method of detecting polymorphism for assessing genetic diversity between genotype and help in the selection of parent for hybridization.


Keywords: Glycine max, RAPD marker, Unweighted pair group method for arithmetic mean (UPGM), Genetic diversity.
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How to cite this article:

Shashank A. Tidke, D. Ramakrishna, S. Kiran, Georgina Kosturkova, Ravishankar, G.A. 2017. Analysis of Genetic Diversity of 12 Genotypes of Glycine max by Using RAPD Marker.Int.J.Curr.Microbiol.App.Sci. 6(7): 656-663. doi: https://doi.org/10.20546/ijcmas.2017.607.080