National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Soybean (Glycine max) is an essential legume crop in developing nations where it serves to meet the increasing demands for protein, edible oil and calories. In this study, we analysed genetic diversity of 12 soybean genotypes using Random Amplified Polymorphic DNA (RAPD) molecular markers. A total of 10 RAPD primers were used for the screening of 12germplasm line out of which 525 fragments were amplified with 10 random primers and 74.85 % were polymorphic. Genetic similarity matrix based on Jaccard Similarity Coefficients of soybean genotypes ranged from 0.30 to 0.77. These coefficients were used to construct a dendrogram using the unweighted pair group of arithmetic means (UPGMA). All 12 soybean genotypes were grouped into six clusters. The largest cluster consisted of 5 genotypes. The highest similarity among the soybean varieties were observed between JS-02-21 and DSB-61 (0.756). Present study indicated a great deal of germplasm diversity among these 12 genotypes. Our study revealed that RAPD technology is potentially simple, rapid, reliable and an effective method of detecting polymorphism for assessing genetic diversity between genotype and help in the selection of parent for hybridization.
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