National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Development of an efficient plant regeneration protocol is the pre-requisite for crop improvement through biotechnological approach. Hence, the in vitro regeneration was achieved from hypocotyl explants. The explants were taken from aseptically raised seedlings of popular Capsicum F1 hybrids Bharat and Indra. Seeds of both hybrids were soaked in distilled water along with GA3 at 2 mg/l to get optimum germination and decontaminated prior to in vitro sowing on half-strength MS medium. Tissue culture responses to morphogenesis varied with the genotypes and combinations of growth regulators used. Per cent regeneration (44.44 %), number of shoots per explants (2.11) and per cent elongation (66.85 %) was maximum in hybrid Indra from hypocotyl explants in medium supplemented with zeatin at 7.5 mg/l along with GA3 at 2.0 mg/l. Cent per cent rooting, optimum number of roots (27.56 and 23.65, respectively) and root length (4.94 cm and 7.71 cm, respectively) were observed when regenerated shoots were cultured on MS media supplemented with 0.5 mg/l IBA in both the hybrids Indra and Bharat. The regenerated plantlets were hardened in pots filled with a sterile mixture of coco-peat and vermiculite (1:1) and thereafter transferred to pots containing soil. The Survival percentage was 85.7% to 92.3% in hybrids Bharat and Indra, respectively.
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