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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(7): 545-557
DOI: https://doi.org/10.20546/ijcmas.2017.607.066


Plant Regeneration from Hypocotyl Explants in Capsicum (Capsicum annuum L.)
Vivek Hegde1*, P.S. Partap1 and R.C. Yadav2
1Department of Vegetable Science,
2Department of Molecular Biology and Biotechnology, CCS Haryana Agricultural University, Hisar-125 004, Haryana, India
*Corresponding author
Abstract:

Development of an efficient plant regeneration protocol is the pre-requisite for crop improvement through biotechnological approach. Hence, the in vitro regeneration was achieved from hypocotyl explants. The explants were taken from aseptically raised seedlings of popular Capsicum F1 hybrids Bharat and Indra. Seeds of both hybrids were soaked in distilled water along with GA3 at 2 mg/l to get optimum germination and decontaminated prior to in vitro sowing on half-strength MS medium. Tissue culture responses to morphogenesis varied with the genotypes and combinations of growth regulators used. Per cent regeneration (44.44 %), number of shoots per explants (2.11) and per cent elongation (66.85 %) was maximum in hybrid Indra from hypocotyl explants in medium supplemented with zeatin at 7.5 mg/l along with GA3 at 2.0 mg/l. Cent per cent rooting, optimum number of roots (27.56 and 23.65, respectively) and root length (4.94 cm and 7.71 cm, respectively) were observed when regenerated shoots were cultured on MS media supplemented with 0.5 mg/l IBA in both the hybrids Indra and Bharat. The regenerated plantlets were hardened in pots filled with a sterile mixture of coco-peat and vermiculite (1:1) and thereafter transferred to pots containing soil. The Survival percentage was 85.7% to 92.3% in hybrids Bharat and Indra, respectively.


Keywords: In vitro regeneration, Hypocotyl, Capsicum, Tissue culture.
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How to cite this article:

Vivek Hegde, P.S. Partap and Yadav, R.C. 2017. Plant Regeneration from Hypocotyl Explants in Capsicum (Capsicum Annuum L.).Int.J.Curr.Microbiol.App.Sci. 6(7): 545-557. doi: https://doi.org/10.20546/ijcmas.2017.607.066