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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(4): 68-83
DOI: http://dx.doi.org/10.20546/ijcmas.2016.504.011


Detection of Rotavirus A and Escherichia coli from Diarrhea Cases in Children and Coliphage Characterization
Marwa M. Yaqoob1, Kuther H. Mahdi1, Hayder Abdulhussein Al-Hmudi1* Detection of Rotavirus A and Escherichia coli from Diarrhea Cases in Children and Coliphage Characterization
and Mariem N. Mohammed-Ali2
1College of Science, University of Basra, Basra, Iraq
2College of Medicine, University of Basra, Basra, Iraq
*Corresponding author
Abstract:

Acute gastroenteritis is a common disorder in young children. The purpose of this study was to comprehensive determination of main diarrheagenic pathotypes in children with acute gastroenteritis in the pediatric population in Basra city/Iraq, and characterization of E.coli phag. This study determined Rotavirus A and bacterial pathogens in 300 stool samples of children by using different techniques. In our study among children with gastroenteritis was 93/300 (31%) Rotavirus positive cases by Immunochromatographic (IC) test as monoinfection, coinfection, and mixing infections. Out of 50 IC positives fecal samples were tested using EM, 50(100%) were found positive. A total of 80 stools were examined for Rotavirus using polyacrylamide gel electrophoresis. The overall agreement was 68/80(85%). Out of 277/300 (92.33%) bacterial pathogens isolated, 163 (54.33%) children had infections with EPEC Escherichia coli, 39/300   (13%) cases with Salmonella spp., While, Shigella spp. was reported in 12/300 (4.01%) samples. Also parasitic causes were found in 6/300 (2%) samples. Coinfection with another pathogen was observed in 109/300 (36.34%) cases, coinfection with Rotavirus and EPEC Escherichia coli were the most common and occurred in 75/300 (25%). The phage ɸEC-MH1 was isolation successfully from sewage. The phage titer was determined by serial dilution (10-1, 10-2, 10-3, 10-4, 10-5, 10-6, 10-7, 10-8, 10-9) of the sample by  counting  the  number  of plaque  forming  units  (p.f.u.)  for  each dilution. Our results revealed that dilution factor 10-2 was the best countable number of plaques. Effects of chloroform on phage titer during different times was completely inactivated, while sensitivity to saline environments was 3.0*10-4, 4.2*10-4, 4.2*10-4, 5.6*10-4, 6.0*10-4, 6.7*10-4, 8.2*10-4, 8.0*10-4, and 8.4*10-4 during 5,10, 15, 20, 25,  30, 35 and 40 minutes. The  statistical  analysis  was  significantly  decrease P 0.05  in  phage  titer at  the  temperature 50C°  and  65C° comparing  with  phage  titer at  the  temperature 37C°. We concluded that Rotavirus  A could  be  diagnosed  in  stool  samples  of children  with  gastroenteritis  by  IC test  as  a  rapid  technique.  Rotavirus and EPEC Escherichia coli were the most common coinfectious agents responsible for gastroenteritis.


Keywords: Acute gastroenteritis, Rotavirus, Escherichia coli, Coliphage.
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How to cite this article:

Marwa M. Yaqoob, Kuther H. Mahdi, Hayder Abdulhussein Al-Hmudi and Mariem N. Mohammed-Ali. 2016. Detection of Rotavirus A and Escherichia coli from Diarrhea Cases in Children and Coliphage CharacterizationInt.J.Curr.Microbiol.App.Sci. 5(4): 68-83. doi: http://dx.doi.org/10.20546/ijcmas.2016.504.011