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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Acute gastroenteritis is a common disorder in young children. The purpose of this study was to comprehensive determination of main diarrheagenic pathotypes in children with acute gastroenteritis in the pediatric population in Basra city/Iraq, and characterization of E.coli phag. This study determined Rotavirus A and bacterial pathogens in 300 stool samples of children by using different techniques. In our study among children with gastroenteritis was 93/300 (31%) Rotavirus positive cases by Immunochromatographic (IC) test as monoinfection, coinfection, and mixing infections. Out of 50 IC positives fecal samples were tested using EM, 50(100%) were found positive. A total of 80 stools were examined for Rotavirus using polyacrylamide gel electrophoresis. The overall agreement was 68/80(85%). Out of 277/300 (92.33%) bacterial pathogens isolated, 163 (54.33%) children had infections with EPEC Escherichia coli, 39/300 (13%) cases with Salmonella spp., While, Shigella spp. was reported in 12/300 (4.01%) samples. Also parasitic causes were found in 6/300 (2%) samples. Coinfection with another pathogen was observed in 109/300 (36.34%) cases, coinfection with Rotavirus and EPEC Escherichia coli were the most common and occurred in 75/300 (25%). The phage ɸEC-MH1 was isolation successfully from sewage. The phage titer was determined by serial dilution (10-1, 10-2, 10-3, 10-4, 10-5, 10-6, 10-7, 10-8, 10-9) of the sample by counting the number of plaque forming units (p.f.u.) for each dilution. Our results revealed that dilution factor 10-2 was the best countable number of plaques. Effects of chloroform on phage titer during different times was completely inactivated, while sensitivity to saline environments was 3.0*10-4, 4.2*10-4, 4.2*10-4, 5.6*10-4, 6.0*10-4, 6.7*10-4, 8.2*10-4, 8.0*10-4, and 8.4*10-4 during 5,10, 15, 20, 25, 30, 35 and 40 minutes. The statistical analysis was significantly decrease P≤ 0.05 in phage titer at the temperature 50C° and 65C° comparing with phage titer at the temperature 37C°. We concluded that Rotavirus A could be diagnosed in stool samples of children with gastroenteritis by IC test as a rapid technique. Rotavirus and EPEC Escherichia coli were the most common coinfectious agents responsible for gastroenteritis.