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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(7): 499-509
DOI: https://doi.org/10.20546/ijcmas.2017.607.060


Antifungal Resistance Pattern among Dermatophytes in Western Rajasthan
Prabhat Kiran Khatri1*, Dileep Kachhawa1, Vinod Maurya1, Saroj Meena2, Archana Bora2, Laxmi Rathore2, K.L. Seervi2 and Shivani Khullar2
1Department of Microbiology,
2Department of Skin and VD, Dr. S. N. Medical College, Jodhpur, Rajasthan, India
*Corresponding author
Abstract:

Dermatophytosis is a major health related problem in areas with high humidity, overpopulation and poor hygienic conditions. Treatment options are topical as well as systemic antifungal drugs. But during course of time dermatophytes have also evolved drug resistance for single as well as multiple drugs simultaneously. Therefore it became necessary to find out antifungal susceptibility for dermatophytes. In this study we are using disc diffusion method for antifungal susceptibility testing. To find out antifungal resistance pattern among dermatophytes. 80 patients of all age group with clinical diagnosis of dermatophytosis were included under study. Skin, hair and nail samples were taken aseptically. These samples were subjected to KOH mount and culture on SDA. For sensitivity testing mature colony was subcultured on PDA and after significant sporulation, spores were harvested in normal saline and standardised inoculum of 1x106cfu/ml was streaked over MHA plated and incubated at 28ËšC. Zone of inhibition was measured after 3-7days and were classified as sensitive, intermediate or resistant for a particular drug. Resistance against fluconazole and terbinafine was most common, 61.33% and 48% respectively. Resistance against voriconazole and miconazole was not observed in this study. Resistance against fluconazole was noted among all species of dermatophytes, followed by terbinafine and clotrimazole. Fluconazole was least effective drug followed by terbinafine. voriconazole and miconazole were more effective. Due to increasing trend of resistance among dermatophytes treatment should be based on antifungal sensitivity testing.


Keywords: Dermatophytosis, Antifungal Susceptibility Testing, Disc Diffusion Method, Western Rajasthan.
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How to cite this article:

Prabhat Kiran Khatri, Dileep Kachhawa, Vinod Maurya, Saroj Meena, Archana Bora, Laxmi Rathore, K.L. Seervi and Shivani Khullar. 2017. Antifungal Resistance Pattern among Dermatophytes in Western Rajasthan.Int.J.Curr.Microbiol.App.Sci. 6(7): 499-509. doi: https://doi.org/10.20546/ijcmas.2017.607.060