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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(7): 449-461
DOI: https://doi.org/10.20546/ijcmas.2017.607.054


Molecular Detection of Oral Veillonella Species in the Saliva of Children with Different Oral Hygiene Statuses
Citra Fragrantia Theodorea1,2, Izumi Mashima1,3,4, Boonyanit Thaweboon5, Sroisiri Thaweboon5 and Futoshi Nakazawa1*
1Department of Oral Microbiology, School of Dentistry, Health Sciences University of Hokkaido, 1757 Kanazawa, Ishikari-Tobetsu, Hokkaido, 061-0293, Japan
2Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Salemba Raya No.4, Jakarta Pusat 10430, Indonesia
3Postdoctoral Fellow of Japan Society for the Promotion of Science, 5-3-1, Kouji-machi, Chiyoda-Ku, Tokyo, 102-0083, Japan
4Department of Oral Biology, School of Dental Medicine, University at Buffalo, The State University of New York, 3435 Main Street, 109 Foster Hall, Buffalo, New York 14214, USA
5Department of Oral Microbiology, Faculty of Dentistry, Mahidol University, 6 Yothi Street, Bangkok, 10400, Thailand
*Corresponding author
Abstract:

This study examined the distribution and frequency of oral Veillonella species in the saliva of 107 Thai children with different oral hygiene statuses (good, moderate, and poor). A total of 1609 Veillonella strains were isolated and confirmed by PCR with genus-specific primers. Oral Veillonella isolates were detected at 2-fold higher frequency in subjects with poor than with good or moderate oral hygiene. Veillonella species were identified by one-step PCR using species-specific primers based on rpoB of oral Veillonella species. Veillonella rogosae prevalence was significantly lower in the poor oral hygiene group than in the good oral hygiene groups. Veillonella parvula, V. tobetsuensis, and the unclassified Veillonella isolate were significantly more prevalent in the poor oral hygiene group. Veillonella tobetsuensis was not detected in the good oral hygiene group. Thus, the detection rate of oral Veillonella species such as V. rogosae, V. parvula, and V. tobetsuensis in the saliva indicates the oral hygiene status of children. This is the first report indicating an association between the distribution and frequency of oral Veillonella species in saliva and oral hygiene status of children. Other Veillonella species and novel species of the genus Veillonella may inhabit the oral cavity of children.


Keywords: Oral Veillonella species, Saliva, Children, Oral hygiene status, One-step PCR.
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How to cite this article:

Citra Fragrantia Theodorea, Izumi Mashima, Boonyanit Thaweboon, Sroisiri Thaweboon and Futoshi Nakazawa. 2017. Molecular Detection of Oral Veillonella Species in the Saliva of Children with Different Oral Hygiene Statuses.Int.J.Curr.Microbiol.App.Sci. 6(7): 449-461. doi: https://doi.org/10.20546/ijcmas.2017.607.054