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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(7): 161-167
DOI: https://doi.org/10.20546/ijcmas.2017.607.019


Cloning of Major Surface Antigen (SAG1) Gene of Toxoplasma gondii Tachyzoites
Deepali G. Kalambheand J.P.S. Gill
School of Public Health and Zoonoses, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141004, India
*Corresponding author
Abstract:

Toxoplasma gondii is an obligate intracellular protozoon parasite of all the warm blooded animals. Members of feline family are the definitive hosts whereas other warm blooded animals are the intermediate hosts. Serological assays employing whole cell antigens are widely used for diagnosis of toxoplasmosis. Availability of stage specific antigen increases the specificity of a test in accurate detection of infection. This study was aimed at cloning of tachyzoites stage specific SAG1 gene of T. gondii to make availability of specific Toxoplasma antigen for accurate detection of acute infection. T. gondii SAG1 gene was amplified from the tachyzoite DNA using the published set of primers and amplicon was cloned in the pPROEXHTc cloning cum expression vector after RE double digestion of insert and vector. The results showed that immunodominant SAG1gene of Toxoplasma tachyzoites was successfully cloned in pPROEXHTc expression vector which was confirmed by PCR and sequencing for the presence of insert in the recombinant plasmid and thus it is ready for further recombinant protein expression studies.


Keywords: Toxoplasma tachyzoite, SAG1, Cloning, pPROEXHTc expression vector
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How to cite this article:

Deepali G. Kalambhe and Gill, J.P.S. 2017. Cloning of Major Surface Antigen (SAG1) Gene of Toxoplasma gondii Tachyzoites.Int.J.Curr.Microbiol.App.Sci. 6(7): 161-167. doi: https://doi.org/10.20546/ijcmas.2017.607.019