National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Toxoplasma gondii is an obligate intracellular protozoon parasite of all the warm blooded animals. Members of feline family are the definitive hosts whereas other warm blooded animals are the intermediate hosts. Serological assays employing whole cell antigens are widely used for diagnosis of toxoplasmosis. Availability of stage specific antigen increases the specificity of a test in accurate detection of infection. This study was aimed at cloning of tachyzoites stage specific SAG1 gene of T. gondii to make availability of specific Toxoplasma antigen for accurate detection of acute infection. T. gondii SAG1 gene was amplified from the tachyzoite DNA using the published set of primers and amplicon was cloned in the pPROEXHTc cloning cum expression vector after RE double digestion of insert and vector. The results showed that immunodominant SAG1gene of Toxoplasma tachyzoites was successfully cloned in pPROEXHTc expression vector which was confirmed by PCR and sequencing for the presence of insert in the recombinant plasmid and thus it is ready for further recombinant protein expression studies.
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