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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(6): 3258-3274
DOI: https://doi.org/10.20546/ijcmas.2017.606.384


Fecal calprotectin among patients infected with some protozoan infections
Yahya Jirjees Salman1*, Chateen Aizideen Ali2 and Adil Abdu Allah Abdul Razaq1
1Department of Microbial, Kirkuk Faculty of Medicine, Iraq
2Department of Basic science, College of Dentistry, Tikrit University, Iraq
*Corresponding author
Abstract:

Intestinal parasitic infections have had a strong impact on people with gastro-intestinal tract disorders (GITDs) particularly those with irritable bowel syndrome (IBS) and inflammatory disease (IBD). Fecal calprotectin g(FC) test had a role in detecting the causes of GITDs as indicated to the damages caused by the infectious agents to the host; particularly the parasitic infections. The aim of the current study was to assess a relationship between parasitic infection and fecal calprotecting. To achieve that, 266 patients were chosen by the gasto-enterologist in two main Hospitals in Kirkuk city, they were referred for general stool examination and to determine fecal calprotectin. Direct microscopy was used in preparation of double wet amount of 0.9 % of NaCl and 1 % of Lugols iodine. Whereas for FC special ELISA sandwich kit was applied. The overall rate of parasitic infection was 64.28 % in 171 stool samples, this rate was contributed five following protozoan parasites: Entamoeba histolytica Giardia lamblia, Blastocystis hominis, Dientamoeba fragilis and Chilomastix mesnili, the rates were: 94 (34.21 %), 47(17.66 %), 31(11.65 %), 1(0.37 %) and 1(0.37 %) respectively. Eighty one patients (30.45 %) have IBD versus to 43(16.16 %) for IBS; high rates 43.95%, 57,44%and 38.70 % have: Entamoeba histolytica Giardia lamblia, Blastocystis hominis among IBD were recorded compared to 27.47 %, 21.27 % and 25.80% respectively, P<0.05. Fecal calprotectin positive mean levels (above than 50 ng/ml) were found as followings: 38(41.75 %) for Entamoeba histolytica, followed by 10(21.27 %) for Giardia lamblia and 1(3.22 %) for Blastocystis hominis; whereas FC levels were at negative level for Dientamoeba fragilis and Chilomastix mesnili. Co-existence of Helicobacter pylori (H. pylori) was seen with protozoan infections, 122 (71.34 %), with high dominant incidence 38(41.45 %) was for Entamoeba histolytica. According to patients age high mean level 86.89 ng/ml and 51.75ng/ml of FC were found among patients aging above than 70 years and those between 61 to 69 years. Correlation among FC, protozoan infections, white blood cell total count and neutrophil % was significant, P<0.05. Relationship between patient's occupation and positive level of FC was significant, high rates 46.05 % and 41.86 % were recorded among house makers and workers respectively. The rate of intestinal protozoan infections among patients with GITDs was high. Fecal calprotectin has had high efficacy for revealing the damages in GIT due toprotozoan infections and H. pylori infections among patients with GITDs.


Keywords: Calprotectin, ELISA, Giardia, Dientamoeba, Neutrophils
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How to cite this article:

Yahya Jirjees Salman, Chateen Aizideen Ali and Adil Abdu Allah Abdul Razaq. 2017. Fecal calprotectin among patients infected with some protozoan infections.Int.J.Curr.Microbiol.App.Sci. 6(6): 3258-3274. doi: https://doi.org/10.20546/ijcmas.2017.606.384