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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 6, Issue:6, June, 2017

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(6): 3220-3226
DOI: https://doi.org/10.20546/ijcmas.2017.606.379


Variability in Protein and Esterase Profiles of Botryodiplodia theobromae an Incitant of Longitudinal Splitting of Bark and Wood Disease of Acidlime (Citrus aurantifolia Swingle)
T. Gouri Sankar, V. Gopi, K. Gopal, Y. Hema Bharathi and Y. Sreenivasulu
AICRP on Fruits (Citrus), Citrus Research Station, Dr. Y.S.R. Horticultural University, Tirupati 517 502 (A.P), India
*Corresponding author
Abstract:

Biochemical studies on variability in protein and esterase profiles between twelve isolates of Botryodiplodia theobromae causing Longitudinal Splitting of Bark and Wood Disease (LBWP) on acid lime reveled that isolates LBWP-1 and LBWP-5 exhibited protein pattern with eight banding loci each, LBWP-4 and LBWP-6 with seven banding loci, LBWP-7, LBWP-8 and LBWP-9 with five banding loci and the isolates LBWP-3, LBWP-10, LBWP-11 and LBWP-12 with four banding loci, LBWP-2 exhibited protein pattern with six banding loci. LBWP-1 isolate found to be unique in having one band with Rm value of 0.83 which was absent in other isolates. Considerable difference among the isolates in banding loci of proteins and considerable variation among the isolates in banding loci of esterase enzyme were noticed. LBWP-5 found to be unique in having two bands with Rm values of 0.18 and 0.32 which were absent in other isolates. Apart from this all the isolates under the study showed significant quantitative differences in the banding profiles for mycelial esterase.


Keywords: Variability, Biochemical, Protein, Enzyme

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How to cite this article:

Gouri Sankar, T., V. Gopi, K. Gopal, Y. Hema Bharathi and Sreenivasulu, Y. 2017. Variability in Protein and Esterase Profiles of Botryodiplodia theobromae an Incitant of Longitudinal Splitting of Bark and Wood Disease of Acidlime (Citrus aurantifolia Swingle).Int.J.Curr.Microbiol.App.Sci. 6(6): 3220-3226. doi: https://doi.org/10.20546/ijcmas.2017.606.379
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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