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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(6): 1834-1845
DOI: https://doi.org/10.20546/ijcmas.2017.606.214


Green Synthesis of Metallic Nanoparticles using Aqueous Plant Extract and their Antibacterial Activity
S. Saranya1*, A. Eswari3, E. Gayathri3, S. Eswari2 and K. Vijayarani1
1Department of Animal Biotechnology, 2Department of Veterinary Physiology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai-600007, India 3Department of Biotechnology, St. Joseph's College of Engineering, Chennai-600119, India
*Corresponding author
Abstract:

Plant extracts from Musa ornate and Zea mays were used for the green synthesis of Copper (Cu) and Zinc oxide (ZnO) nanoparticles (NPs) from copper chloride and zinc sulphate solution respectively. Green synthesized metallic nanoparticles were characterized by UV–visible spectrophotometer, X-ray diffractometer (XRD), Fourier Transform Infra-Red spectrophotometer (FTIR), Scanning Electron Microscope (SEM), Transmission Electron Microscope (TEM), Atomic Force Microscope (AFM) and Zeta potential particle size analyser. Optimum parameters such as precursor salt solution concentration, pH, ratio between reducing agent and precursor salt solution and reaction time, the formation and stability of the reduced metal nanoparticles in the colloidal solution were monitored by UV–visible spectrophotometer analysis. The mean particle diameter of nanoparticles was calculated from the XRD pattern according to the line width of the plane, refraction peak using the Scherrer’s equation. FTIR results suggested that possible biomolecules for the reduction of metallic nanoparticles. SEM and TEM analysis showed the formation of well dispersed metallic nanoparticles and the synthesized metallic nanoparticles were in nano scale range. Antimicrobial activities of the metallic nanoparticles were performed by well diffusion method against Escherichia coli, Staphylococcus aureus, Streptococcus agalactiae and Salmonella enterica. Metallic Cu and ZnO NPs synthesized had antimicrobial activity against pathogenic bacteria and highest antimicrobial activity was found with Cu NPs synthesized using Musa ornate flower sheath against Staphylococcus agalactiae.


Keywords: Copper, Zinc oxide, Musa ornate, Zea mays, Metallic nanoparticles, Antibacterial activity, Well diffusion method
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How to cite this article:

Saranya, S., A. Eswari, E. Gayathri, S. Eswari and Vijayarani, K. 2017. Green Synthesis of Metallic Nanoparticles using Aqueous Plant Extract and their Antibacterial Activity.Int.J.Curr.Microbiol.App.Sci. 6(6): 1834-1845. doi: https://doi.org/10.20546/ijcmas.2017.606.214