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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(6): 1792-1797
DOI: https://doi.org/10.20546/ijcmas.2017.606.208


Isolation and Molecular Identification of Aspergillus spp. Collected from Different Sources of Animals Feed
Ahmed D. Ahmed1*, Nazar J. Al-Khafaji1 and Luma T. Ahmed2
1Branch of Microbiology College of Veterinary Medicine, University of Diyala, Diyala, Iraq 2Branch of Microbiology College of Medicine, University of Diyala, Diyala, Iraq
*Corresponding author
Abstract:

Aspergillus spp. is one of the most important fungal spp. In the mycology kingdom and has many important medical, industrial and commercial interesting, it's one of the essential contaminants of food and feed especially under specific circumstances. Aspergillus species was isolated with other fungal species from different samples of feed used by the farmers in different places in the Diyala province such as Fusarium, Aspergillus niger, Aspergillus terrus, Penicillium species and purified in the especial agar media then DNA was successfully isolated from the Aspergillus spp. by using of a commercial kit. PCR was done by using two different primers NS1, C18 and ITS1, ITS4. The first primer sequence didnít give any result while the second primer revealed the amplification band of all isolated Aspergillus which improve the presence of the a flat oxigenic Aspergillus species in the 10 different feed samples.


Keywords: Compound feed, Aspergillus species, Molecular identification
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How to cite this article:

Ahmed D. Ahmed, Nazar J. Al-Khafaji and Luma T. Ahmed. 2017. Isolation and Molecular Identification of Aspergillus spp. Collected from Different Sources of Animals Feed.Int.J.Curr.Microbiol.App.Sci. 6(6): 1792-1797. doi: https://doi.org/10.20546/ijcmas.2017.606.208