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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(6): 1222-1228
DOI: https://doi.org/10.20546/ijcmas.2017.606.142


Comparative Assessment of Seller's Staining Test (SST), Direct Fluorescent Antibody Test and Real Time PCR for Diagnosis of Rabies in Dogs
R.R. Pharande1*, S.B. Majee1, A.S. Bannalikar1, R.S. Gandge1, R.V. Dighe1, S.S. Moregoankar1, A.Y. Doiphode1, P. Charan2 and S. Mukherjee2
1Department of Microbiology, Bomabay Veterinary College, Parel, Mumbai-400012, India 2Department of Virology and Immunology, Haffkine Institute, Parel, Mumbai, India
*Corresponding author
Abstract:

Rabies is a highly fatal viral infection of central nervous system affecting all warm blooded animals including humans. The virus belongs to the Rhabdoviridae family, genus Lyssa virus. Several techniques have been standardized internationally for the diagnosis of rabies. This study was designed to assess the efficacy of Seller's staining test (SST) and Direct Fluorescent Antibody Test (dFAT) for diagnosis of rabies antigen, Mouse Innoculation Test (MIT) and real time reverse transcriptase polymerase chain reaction (qRT-PCR) were used as the Gold Standards. Antibodies against the N Nucleoprotein were used for the dFAT, whereas the nucleoprotein gene was targeted in the qRT-PCR assay. A total of 10 rabies suspected dog brain samples received at the Department of Microbiology at Bombay Veterinary College, Mumbai, were tested by Seller's staining test (SST), Direct Fluorescent Antibody Test (dFAT) and Mouse Innoculation Test (MIT) as per the protocol described by WHO (2007). Out of 10 brain specimens Negri bodies were observed in 05 (50%) brain samples by SST. dFAT tested positive for 06 (60%) samples, rabies virus was isolated in 07 samples by intra-cerebral inoculation in Swiss-albino mice and 07 (70%) samples positive for rabies using qRT-PCR. Compared to the qRT-PCR, the sensitivities of SST and dFAT were 71.42 % and 85.71%, respectively. It was concluded that qRT-PCR is a more sensitive and better molecular approach for diagnosis of rabies from brain of rabid suspected cases as compare to SST and dFAT.


Keywords: Rabies, Seller's staining test, Direct fluorescent antibody test, Real time reverse transcriptase polymerase chain reaction
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How to cite this article:

Pharande, R.R., S.B. Majee, A.S. Bannalikar, R.S. Gandge, R.V. Dighe, S.S. Moregoankar, A.Y. Doiphode, P. Charan and Mukherjee, S. 2017. Comparative Assessment of Seller's Staining Test (SST), Direct Fluorescent Antibody Test and Real Time PCR for Diagnosis of Rabies in Dogs.Int.J.Curr.Microbiol.App.Sci. 6(6): 1222-1228. doi: https://doi.org/10.20546/ijcmas.2017.606.142