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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(6): 3132-3149
DOI: https://doi.org/10.20546/ijcmas.2017.606.370


Molecular and Biochemical Characterization of Potential Isolates of Trichoderma Species Effective against Soil-Borne Pathogens
N. Srinivasa1*, Deeba Kamil1, Chandu Singh, Avinash Singode3 and Deeksha Gupta1
1Division of Plant Pathology, 2Seed Production Unit, ICAR-Indian Agricultural Research Institute, New Delhi, India
4ICAR-Indian Institute of Millet Research, Hyderabad, India
*Corresponding author
Abstract:

Trichoderma species is one of the key potential bio-control agents against soil-borne pathogens. In this study molecular and biochemical characterization were done using twenty four potential isolates of Trichoderma species, based on internal transcribed spacer (ITS 1 & 4), translation elongation factor(tef-1) gene region and hydrolytic enzymes. In this studytef-1 was found to be better than ITS, to distinguish the Trichoderma isolates into two different species viz., Trichoderma virens and Trichoderma harzianum, on the basis of maximum parsimony sequence analysis. The specific activity of the hydrolytic enzymes showed the significance difference between both the species of Trichoderma, tested against three different pathogens such as Fusarium oxysporum, Rhizoctonia solani and Sclerotiumrolfsii. It was also found that cultivation of Trichoderma isolates with soil borne pathogen (during interaction) produced high hydrolytic enzymes compared to Trichoderma species alone. Among the potential isolates tested for enzyme assay, three isolates viz., V-7, V-19 and V-21 of T. virens and three isolates such as H-10, H-12 and H-21 of T. harzianum were found as high potential isolates based on its specific activity of the hydrolytic enzymes. Therefore, the identified isolates could be effectively used as potential bio-control agents against soil-borne plant pathogens.


Keywords: Biochemical, Molecular, Hydrolytic enzymes, Trichoderma harzianum, Trichoderma virens, Soil-borne pathogens.
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How to cite this article:

Srinivasa, N., Deeba Kamil, Chandu Singh, Avinash Singode and Deeksha Gupta. 2017. Molecular and Biochemical Characterization of Potential Isolates of Trichoderma Species Effective against Soil-Borne Pathogens.Int.J.Curr.Microbiol.App.Sci. 6(6): 3132-3149. doi: https://doi.org/10.20546/ijcmas.2017.606.370