Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(6): 3098-3112
DOI: https://doi.org/10.20546/ijcmas.2017.606.367


Influence of Culture Media and Temperature on Growth and Sporulation of Lasiodiplodia theobromae, Pestalotiopsis microspora and Fusarium oxysporum Isolated from Ricinodendron heudelotii in Cameroon
Joseph Djeugap Fovo1*, Daniel Dostaler2 and Louis Bernier3
1Phytopathology Laboratory, Department of Plant Protection, Faculty of Agronomy and Agricultural Science, University of Dschang, Box 222 Dschang, Cameroon
2Plant Pathology Laboratory, Department of Phytology, Faculty of Agricultural Science and Food, Université Laval, G1V 0A6 Qc, Québec, Canada
3Forest Pathology Laboratory, Department of Wood science and Forest, Faculty of Forestry, Geography and Geomatic, Université Laval, G1V 0A6 Qc, Québec, Canada
*Corresponding author
Abstract:

The effects of mycological media and temperature on mycelial growth and spore production of three fungal pathogens recently reported on Ricinodendron heudelotii were investigated. The pathogens were identified on the basis of the ITS sequences of their ribosomal DNA as Pestalotiopsis microspora (isolate PMHP_109L), Lasiodiplodia theobromae (isolate LTHP_110L) and Fusarium oxysporum (isolates FOBR_164S, FOBR_049L and FOHP_121L). The radial growth (mm/day) of the fungi and conidia concentrations (number of conidia/ml of suspension) were assessed on three culture media: potato dextrose agar (PDA), malt extract agar (MEA) and V8 juice agar (V8). All media were suitable for the growth of L. theobromae while V8 juice agar supported the fastest mycelial growth rate in P. microspora (13.4 mm/day). PDA and MEA media were appropriate for F. oxysporum. The growth rate and conidia concentration increased with temperature and attained their optimum at 23oC for P. microspora and L. theobromae and 28oC for Fusarium oxysporum strains. There was no growth of P. microspora and L. theobromae at 33oC while at this temperature, Fusarium oxysporum strains continued to grow and produce spores. The best temperature for spore production was 23oC for P. microspora, 28oC for F. oxyporum and 21, 23 and 28oC for L. theobromae. F. oxysporum isolates produced the highest concentration of conidia in all the culture media. These data contribute to the knowledge of the biology of these newly recognized parasitic fungi on R. heudelotii and show that species like F. oxysporum possesses a high level of phenotypic plasticity that allows it to survive and proliferate over a wide range of environmental conditions.


Keywords: Pestalotiopsis microspora, Lasiodiplodia theobromae, Fusarium oxysporum, Culture media, Temperature, Mycelial growth, Sporulation, Ricinodendron heudelotii.
Download this article as Download

How to cite this article:

Joseph Djeugap Fovo, Daniel Dostaler and Louis Bernier. 2017. Influence of Culture Media and Temperature on Growth and Sporulation of Lasiodiplodia theobromae, Pestalotiopsis microspora and Fusarium oxysporum Isolated from Ricinodendron heudelotii in Cameroon.Int.J.Curr.Microbiol.App.Sci. 6(6): 3098-3112. doi: https://doi.org/10.20546/ijcmas.2017.606.367