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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(6): 2744-2759
DOI: https://doi.org/10.20546/ijcmas.2017.606.328


Enhancement of Phytase Production from a New Probiotic Strain Bacillus subtilis P6
Shraddha Trivedi, Anjana Sharma* and Prakash Jain
Bacteriology Laboratory, Division of P.G. Studies and Research in Biological Science, Rani Durgavati University, Jabalpur-482004, Madhya Pradesh, India
*Corresponding author
Abstract:

An integrated classical and statistical optimization approach involving the combination of Placket–Burman design (PBD) and Central Composite Design (CCD) was employed for increasing phytase yield. PBD was used to evaluate the effect of 9 variables related to phytase production from probiotic strain B. subtilis, and three statistically significant variables, namely, glucose, beef extract and potassium phosphate were selected for further optimization studies. The levels of five variables for maximum phytase production were determined by a CCD. The optimum values for the factors were determined via response surface methodology (RSM) as: 6.59 gl-1 of glucose, 6 gl-1 of beef extract and 2.75 gl-1 of potassium phosphate respectively. Phytase production improved from 2.74 EUml-1 to 46.76 EUml-1 indicating 17-fold increase in activity after optimization.


Keywords: Phytase, Probiotic, classical optimization, Plackett-Burman Design, Response surface optimization
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How to cite this article:

Shraddha Trivedi, Anjana Sharma and Prakash Jain. 2017. Enhancement of Phytase Production from a New Probiotic Strain Bacillus subtilis P6.Int.J.Curr.Microbiol.App.Sci. 6(6): 2744-2759. doi: https://doi.org/10.20546/ijcmas.2017.606.328