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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(6): 2500-2505
DOI: https://doi.org/10.20546/ijcmas.2017.606.297


Phytochemical Screening and Antibacterial Activity of Erythrina variegata L. (Leaf Extract)
Preeti Kumari*, Subhankar K. Singh and Chandrawati Kumari
Department of Biotechnology, A.N. College, Magadh University, Patna, Bihar, India
*Corresponding author
Abstract:

Various extracts on the basis of polarity of solvent of Erythrina variegate L. (family- fabaceae) leaves such as hexane, chloroform, dichloromethane, ethyl acetate, methanol and aqueous were evaluated for their phytochemicals and antibacterial activity against selected five bacterial culture viz. Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumoniae, Proteus vulgaris by disc diffusion method. The activities of the samples were compared with that of standard antibiotics e.g. Amikacin. Phyto-chemical screening of Erythrina variegata leaf extract in five different solvents showed the presence of important phyto-constituents like phenols, alkaloids, flavonoids, tannins and saponins. In antibacterial assay, all the organisms respond to the plant extract but inhibitory zone developed. Among the selected solvent used in soxhlet apparatus extract were hexane, chloroform, dichloromethane, ethyl acetate, methanol and aqueous, all developed inhibition zone against bacteria; this means selected plant has antibacterial property. The chloroform extract showed a higher activity than other extracts. The methanolic leaf extract of E. variegate showed higher activity against Pseudomonas aeruginosa and Staphylococcus aureus followed by Klebsiella pneumoniae, Proteus vulgaris and Escherichia coli. The methanolic leaf extract of E. variegata showed maximum zone of inhibition against Staphylococcus aureus. The ethyl acetate extract of E. variegata exhibited high activity against E. coli and Staphylococcus aureus followed by methanol, chloroform, hexane and aqueous extracts. The results revealed that the antimicrobial activity exhibited by E. variegata chloroform and aqueous extract noted to be most effective than other solvents.


Keywords: Antimicrobial activity, Antibiotics, Erythrina variegate L. (Leaves), Phytochemical screening.
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How to cite this article:

Preeti Kumari, Subhankar K. Singh and Chandrawati Kumari. 2017. Phytochemical Screening and Antibacterial Activity of Erythrina variegata L. (Leaf Extract).Int.J.Curr.Microbiol.App.Sci. 6(6): 2500-2505. doi: https://doi.org/10.20546/ijcmas.2017.606.297