Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(6): 2435-2445
DOI: https://doi.org/10.20546/ijcmas.2017.606.289


Validation of RNA Interference for Improvement in Mustard (Brassica sp.): A Review
Geeta Boken1, Baldeep Singh2*, Rupinder Kaur3, Geetika Mehta4, Shabir H. Wani5, A. Haribhushan6 and Telem Ratan Singh6
1Department of Genetics and Plant Breeding, CCS Haryana Agricultural University, Hisar, India
2Department of Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana, India
3Department of Fruit Science, Punjab Agricultural University, Ludhiana, India
4Indian Institute of Wheat and Barley Research, Karnal, Haryana, India
5MRCFC Khudwani, Sher-e-Kashmir University of Agricultural Sciences and Technology, Kashmir, India
6Farm Science Centre (KVK0 Senapati, Manipur, India
*Corresponding author
Abstract:

RNA interference (RNAi), is a powerful technology for discovering the functional genetic sequences and harness the down regulation of expression of gene(s) specifically. To accomplish the modified gene expression for a particular trait, gene silencing viz. co-suppression, post transcriptional gene silencing, virus-induced gene silencing etc can be used. This molecular phenomenon has become a focal point of modern plant biology research across the globe. Thus it has been remarkably used in crop improvement likewise has become a valuable tool for functional genomics in Brassica (Brassica sp.). The rapid adoption of RNAi has replaced previous antisense technology. RNAi has aided in identification of different functions and biological roles of various mustard genes, which are involved in fertility and somatic embryogenesis, resistance to biotic and abiotic stresses and qualitative improvements in oil seed as well as it also have major role in yield and maturity traits. This review article illustrates comparatively appraised the seminal research efforts in previously used antisense approaches and currently applied breakthroughs of RNAi studies in mustard, indeed for functional characterization of mustard genes analyzed the developed RNAi methodologies, achievements, limitations, and future needs.


Keywords: Antisense, Mustard pest control, Oil quality, Gene silencing.
Download this article as Download

How to cite this article:

Geeta Boken, Baldeep Singh, Rupinder Kaur, Geetika Mehta, Shabir H. Wani, A. Haribhushan and Telem Ratan Singh. 2017. Validation of RNA Interference for improvement in Mustard (Brassica sp.): A Review.Int.J.Curr.Microbiol.App.Sci. 6(6): 2435-2445. doi: https://doi.org/10.20546/ijcmas.2017.606.289