National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
RNA interference (RNAi), is a powerful technology for discovering the functional genetic sequences and harness the down regulation of expression of gene(s) specifically. To accomplish the modified gene expression for a particular trait, gene silencing viz. co-suppression, post transcriptional gene silencing, virus-induced gene silencing etc can be used. This molecular phenomenon has become a focal point of modern plant biology research across the globe. Thus it has been remarkably used in crop improvement likewise has become a valuable tool for functional genomics in Brassica (Brassica sp.). The rapid adoption of RNAi has replaced previous antisense technology. RNAi has aided in identification of different functions and biological roles of various mustard genes, which are involved in fertility and somatic embryogenesis, resistance to biotic and abiotic stresses and qualitative improvements in oil seed as well as it also have major role in yield and maturity traits. This review article illustrates comparatively appraised the seminal research efforts in previously used antisense approaches and currently applied breakthroughs of RNAi studies in mustard, indeed for functional characterization of mustard genes analyzed the developed RNAi methodologies, achievements, limitations, and future needs.
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