Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(3): 909-916
DOI: http://dx.doi.org/10.20546/ijcmas.2016.503.105


Thermal Optimization of ?-amylase Production in Brevibacillus sp.
K.Suribabu1* and K.P.J Hemalatha2
1Department Microbiology and Research Centre, Dr. Lankapalli Bullayya Post-graduate College, Visakhapatnam-530 013.A.P, India
2Department Microbiology, Andhra University, Visakhapatnam-530 003.A.P, India
*Corresponding author
Abstract:

Thermostability is a desired characteristic of most of the industrial enzymes. The production of ?-amylases has been thoroughly investigated and observed that it was affected by a variety of physiochemical factors, such as the composition of the growth medium under various temperature parameters with constant pH 7.0. The enzyme production was assayed in submerged fermentation (SmF) condition. The optimum production of ?-amylase by SmF was observed at 40C in Clarks and Lub Medium (2551 ± 1 U/ml), Soybean Casein Digest Medium (920± 0.13 U/ml) and Tryptone Glucose Beef Extract Medium (260 U/ml). The optimum production of ?-amylase by SmF was observed at 250C in Nutrient Broth (270 U/ml). The optimum production of ?-amylase by SmF was observed at 370C in Pikovskaya’s Medium (3371 ± 0.50 U/ml), Tendler's Non-synthetic Medium (761 ± 0.50 U/ml), Amylase Production Medium (580 U/ml) and Soluble Starch Beef Extract Medium (1930 U/ml). The optimum production of ?-amylase by SmF was observed at 500C in Luria Bertain Broth (240 ± 0.12 U/ml) and Yeast extract peptone Glycerol Glucose Medium (265 U/ml). Thermal variability of ?-amylase produced by Brevibacillus borostelensis R1 in various media may have a significant role in many applications like bakery industry, treatment of effluents from sago and rice industry, sewage water treatment, fodder production, laundry industry and textile industry.


Keywords: Brevibacillus borostelensis R1; ?-amylase; Media; Temperature
Download this article as Download

How to cite this article:

Suribabu, K., and Hemalatha, K.P.J. 2016. Thermal Optimization of ?-amylase Production in Brevibacillus sp.Int.J.Curr.Microbiol.App.Sci. 5(3): 909-916. doi: http://dx.doi.org/10.20546/ijcmas.2016.503.105