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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(6): 2346-2351
DOI: https://doi.org/10.20546/ijcmas.2017.606.277


Identification and Purification of Antimicrobial Lectins from Marine Crab Protunus pelagicus (Linneus, 1775)
Selvaraj Chidhambaradhas1*, Thankaiah Selva Mohan2,Stanislaus2 and Mary Josephine punitha1
1Department of Zoology, Centre of Marine Science and Technology, M.S. University, Rajakkamangalam, Tamil Nadu, India
2Department of Zoology, Rani Anna Government College, Tirunelveli -627008,Tamil Nadu, India
*Corresponding author
Abstract:

Lectins are glycoproteins have the ability to bind carbohydrates. They are involved in biological process such as recognition and binding of carbohydrates, interactions of pathogens, apoptosis, etc. Lectins of different carbohydrate specificities are able to promote growth inhibition or death of bacteria and fungi. In the present investigation made to isolation, identification and antimicrobial activity of lectins from haemolymph of P. pelagicus. The haemolymph of Portunuspelagicus contains a protein, portunuspelagicus lectins (PPL). The antimicrobial activity of the PPL was tested against six bacterial strains is Gram positive bacteria Staphylococcus aureus, and Gram negative bacteria Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumonia, Bacillus subtilis and Enterococcus faecalis. Among the six strains the maximum diameter of inhibition zone was recorded in Staphylococcus aureus and Pseudomonas aeroginosa and lowest diameter of inhibition zone was observed in Enterococcus faecalis. It indicates that, the haemolymph of crabs would be a good source of antibacterial agents.


Keywords: Protunus pelagicus Lectins, Klebsiella pneumonia, STI, AMPs, MIC, LBB.
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How to cite this article:

Selvaraj Chidhambaradhas, Thankaiah Selva Mohan and Stanislaus Mary Josephine Punitha. 2017. Identification and Purification of Antimicrobial Lectins from Marine Crab Protunus pelagicus (Linneus, 1775).Int.J.Curr.Microbiol.App.Sci. 6(6): 2346-2351. doi: https://doi.org/10.20546/ijcmas.2017.606.277