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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(6): 2286-2289
DOI: https://doi.org/10.20546/ijcmas.2017.606.270


Demonstration of Osseous Tissue in Quail Embryo by Alizarin Red Technique
K. Iniyah*, S. Jayachitra, K. Balasundaram and K. Rajendran
Department of Veterinary Anatomy, Veterinary College and Research Institute, TANUVAS, Namakkal, Tamil Nadu– 637 002, India
*Corresponding author
Abstract:

The embryonated Namakkal quail eggs were collected from the hatchery at tenth and fourteenth day of incubation. The eggs were opened by standard technique and the embryos were collected and stained by alizarin red staining technique to demonstrate the osseous tissue development. The scapula, coracoid, clavicle and proximal bones of wing were well developed in both tenth and fourteenth day embryos. But the fusion of pelvic girdle with the synsacrum and the distal bones of wing were completely developed only in fourteenth day embryo. The femur, tibia, fibula, tibiotarsus, tarsometatarsus and digits were found like a rod of bones without distinct joints in tenth day embryo. Skull bones, vertebral bones and ribs were fully ossified in fourteenth day quail embryos. But the sternal ossification was not observed by alizarin red staining technique in both the quail embryos. A ring of scleral ossicles in the sclera around the margin of cornea were distinctly observed in both the embryos studied.


Keywords: Alizarin red, Osseous tissue, Quail embryos, Skeletal development.
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How to cite this article:

Iniyah, K., S. Jayachitra, K. Balasundaram and Rajendran, K. 2017. Demonstration of Osseous Tissue in Quail Embryo by Alizarin Red TechniqueInt.J.Curr.Microbiol.App.Sci. 6(6): 2286-2289. doi: https://doi.org/10.20546/ijcmas.2017.606.270