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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(6): 2225-2231
DOI: https://doi.org/10.20546/ijcmas.2017.606.264


Production of Cellulase from Bacillus subtilis under Solid- State Fermentation Using Fiber Wastes of Palmyra Palm
R. Roopa1, M. Charulatha2 and S. Meignanalakshmi2*
1Sree Sastha Institute of Engineering and Technology, Chennai- 25, Tamil Nadu, India
2Department of Animal Biotechnology, Madras Veterinary College, TANUVAS, Chennai-7, Tamil Nadu, India
*Corresponding author
Abstract:

In the present study, the production of cellulase has been carried out using novel natural waste as substrates by Bacillus subtilis isolated from slaughter house waste rumen fluid. Bacillus subtilis was screened for cellulase production by Congo red assay. pH and temperature were optimized. Agricultural wastes such as Hay, fiber waste of palmyra palm and banana bracts were used as a substrate for cellulase production. Substrates with or without pretreatment using NaOH solution were used for solid state fermentation. After 48hrs of fermentation, extracts were drawn and tested for enzyme activity by DNSA method and total protein content by Lowry’s method. Cellulase activity was found to be 0.1519, 0.0759 and 0.05557 µmol ml-1min-1 when fibers of palmyra palm, hay and banana bracts were used as substrates. Raw cellulose content was found to be 37%, 32% and 23.2% in fibers of palmyra palm, hay and banana bracts respectively. Fibers of palmyra palm were found to be having maximum enzyme activity of 0.1519µmol ml-1min-1 at pH 7, at 48hrs of incubation.


Keywords: Cellulase, Bacillus subtilis, Palmyra palm, Solid state fermentation, Agricultural waste
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How to cite this article:

Roopa, R., M. Charulatha and Meignanalakshmi, S. 2017. Production of Cellulase from Bacillus Subtilis under Solid- State Fermentation Using Fiber Wastes of Palmyra Palm.Int.J.Curr.Microbiol.App.Sci. 6(6): 2225-2231. doi: https://doi.org/10.20546/ijcmas.2017.606.264