Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(6): 2155-2162
DOI: https://doi.org/10.20546/ijcmas.2017.606.254


Molecular Detection of Avian Mycoplasmas in Poultry Affected with Respiratory Infections in Haryana (India)
Piyush Tomar1*, Y. Singh1, N.K. Mahajan1, Naresh Jindal1 and Mahavir Singh2
1Department of Veterinary Public Health and Epidemiology, College of Veterinary Sciences
Lala Lajpat Rai University of Veterinary and Animal Sciences (LUVAS), Hisar, India
2College Central Laboratory, College of Veterinary Sciences, Lala Lajpat Rai University of Veterinary and Animal Sciences (LUVAS), Hisar, India
*Corresponding author
Abstract:

Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS) are the most economically significant Mycoplasma pathogen of poultry. The objective of this study was molecular detection of avian Mycoplasmas in poultry affected with respiratory infections in Haryana (India). In this study 92 pooled tissue samples including trachea, lungs and air sacs were collected from 92 different poultry flocks affected with respiratory infections and were undergone mycoplasmological examination by Polymerase chain reaction (PCR). Four sets of primers including a universal primer (16S rRNA) for genus Mycoplasma, two primers for M. gallisepticum (16S rRNA gene and 16S-23S rRNA IGSR) and one for M. synoviae (16S rRNA gene) were employed for amplification of genes of all the 92 DNA samples extracted from tissue samples. Direct detection of avian Mycoplasma in tissue specimen by PCR using 16S rRNA universal primers for genus Mycoplasma followed by MG and MS specific PCR revealed, 34/92(36.9%) Mycoplasma, 25/92(27%) MG and 2/92 (2.1%) MS. This study demonstrated the high prevalence of MG infection in commercial Broilers farms of Haryana. Therefore, the high prevalence and wide distribution of MG infection warrants immediate attention and preventive strategies to minimize economic impact of MG infection. The present study suggests that the PCR assays performed for MG and MS provide a simple, quick and precise tool to specifically detect these organisms from the field samples which are always found to be complicated by other pathogens.


Keywords: Mycoplasma gallisepticum, Mycoplasma synoviae, PCR.
Download this article as Download

How to cite this article:

Piyush Tomar, Y. Singh, N.K. Mahajan, Naresh Jindal and Mahavir Singh. 2017. Molecular Detection of Avian Mycoplasmas in Poultry Affected with Respiratory Infections in Haryana (India).Int.J.Curr.Microbiol.App.Sci. 6(6): 2155-2162. doi: https://doi.org/10.20546/ijcmas.2017.606.254