Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2019) [Effective from January 1, 2019]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(6): 2092-2098
DOI: https://doi.org/10.20546/ijcmas.2017.606.248


Molecular Discrimination of Klebsiella oxytoca using Polymerase Chain Reaction Targeted Polygalacturonase (pehX) Gene
Tamara H. Zedan*
Department of Medical and Molecular Biotechnology, College of Biotechnology, Al-Nahrain University, Baghdad, Iraq
*Corresponding author
Abstract:

This study aimed to molecular diagnosis of Klebsiella oxytoca using polymerase chain reaction. A total of 325 samples were collected from different clinical and environmental sources from six main hospitals in Baghdad province which is capital of Iraq. Out of 282 isolates, fifty six isolates (19.9 %) were isolated and identified as Klebsiella spp., forty isolates (12.3 %) were isolated and identified as K. pneumoniae (36 clinical and 4 environmental), ten isolates (3.5 %) were isolated and identified as Klebsiella terrigena (10 clinical only), three isolates (1.1 %) were isolated and identified as Klebsiella oxytoca and Klebsiella ornitholytica (2 clinical and 1 environmental) for each one. PCR was performed with the primer that target the pehX, Result showed that all the K. oxytoca isolate gave a clear band with a molecular weight 344 bp in size and this gene was not found in other Klebsiella spp. These results suggest that pehX genotype might be a useful marker to promote identification of K. oxytoca in Iraq, and used as routine protocol in public laboratories in Iraq.


Keywords: Klebsiella oxytoca; Molecular diagnosis; PehX
Download this article as Download

How to cite this article:

Tamara H. Zedan. 2017. Molecular Discrimination of Klebsiella oxytoca using Polymerase Chain Reaction Targeted Polygalacturonase (pehX) GeneInt.J.Curr.Microbiol.App.Sci. 6(6): 2092-2098. doi: https://doi.org/10.20546/ijcmas.2017.606.248