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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(3): 865-875
DOI: http://dx.doi.org/10.20546/ijcmas.2016.503.100


Alterations of miR-320 Family Members as a Novel Diagnostic Biomarkers in Peripheral Blood of Schizophrenia Patients
Tihomir Iliev Vachev1,2*, Nikolay Todorov Popov4, Vili Krasteva Stoyanova2,3, Hristo Yordanov Ivanov2 and Danail Savov Minchev1
1Department of Plant Phisyology and Molecular Biology, University of Plovdiv “Paisii Hilendarski”, 24 Tzar Assen Str., Plovdiv, Bulgaria
2Department of Pediatrics and Medical Genetics, Medical Faculty – Plovdiv, 15A Vassil Aprilov St., 4000 Plovdiv, Bulgaria
3University Hospital “St. George” Plovdiv, 66 Peshtersko Shuse Str., Plovdiv 4000, Bulgaria
4State Phsychiatry Hospital Pazardzhik, 28 Bolnichna Str. Pazardzhik, Bulgaria
*Corresponding author
Abstract:

To date, the development of biomarkers of schizophrenia still remains a challenge. Recently, alterations in the expression of microRNAs (miRNAs) from peripheral blood, serum and post-mortem brain tissue have been linked to schizophrenia and other neurodevelopmental disorders. It is well known that miRNAs are expressed by various cell types and can modulate broad physiological functions. On this basis it is assumed that miRNAs could be useful predictive biomarkers for the diagnosis or prognosis of pathological conditions, like schizophrenia. In order to observe an association between miRNAs and schizophrenia, this study was designed to investigate expression profiling of miRNAs in peripheral blood. The aim of this study was to test whether whole blood miR-320 family members display differential expression profile in schizophrenia patients. The relative expression levels of miR-320 members were analyzed by stem-loop qRT-PCR assay in acohort of 30 participants diagnosed with schizophrenia and 25 age- and gender-matched general population controls. The results indicated that miR-320b, miR-320c and miR-320d were expressed substantially higher in schizophrenia patients than in control subjects, and miR-320a showed thegreatest change. Furthermore, a statistically significant difference of mR-320a level was revealed in schizophrenia subjects compared to healthy controls (p =0,0076). Our results suggest that differentially expressed miR-320 family members might be involved in schizophrenia molecular pathways and blood-based miR-320 signature might be able to serve as potential prognostic biomarkers for schizophrenia. The results confirm that the blood-based miRNA profiling is a feasible way to identify biomarkers for schizophrenia.


Keywords: Schizophrenia,MiRNAs,Stem-loop qRT-PCR,Whole blood
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How to cite this article:

Tihomir Iliev Vachev, Nikolay Todorov Popov, Vili Krasteva Stoyanova, Hristo Yordanov Ivanov and Danail Savov Minchev. 2016. Alterations of miR-320 Family Members as a Novel Diagnostic Biomarkers in Peripheral Blood of Schizophrenia Patients.Int.J.Curr.Microbiol.App.Sci. 5(3): 865-875. doi: http://dx.doi.org/10.20546/ijcmas.2016.503.100