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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(6): 1824-1827
DOI: https://doi.org/10.20546/ijcmas.2017.606.212


Speciation of Coagulase Negative Staphylococcal Isolates from Clinically Significant Specimens and their Antibiogram
Mariraj Jeer, Jyoti R. Hundekar* and S. Krishna
Department of Microbiology, Vijayanagara Institute of Medical sciences, Bellary, India
*Corresponding author
Abstract:

Coagulase Negative Staphylococci (CONS) are the indigenous flora of the human skin and mucous membrane. They are usually contaminants, when isolated from clinical specimens. CONS have become important nosocomial pathogens, accounting for 9% of all nosocomial infections. These infections are difficult to treat because of the risk factors and the multiple drug resistance of these organisms. A total of 74 clinically significant CONS isolated from pus, urine, blood, sputum, ear swabs. These isolates were initially identified by colony morphology, Gram staining, catalase test, slide coagulase test, tube coagulase test. After confirming the isolates as CONS, species-level identification was performed by simple, non-expensive conventional methods and antibiotic sensitivity testing was also carried out by Kirby-Buer’s disc diffusion method. Among the 74 isolates, S. epidermidis was the most common species isolated, seen in 29.7%, followed by S. hemolyticus (20.2%), S. saprophyticus (14.8%), S. lugdunensis (13.5%), S. capitis (10.8%), S. cohni (4%), S. schleiferi (2.7%), S. xylosus and S. hominis (1.3% each), Most of the isolates showed resistance to penicillin (83.7%) fallowed by Ampicillin (77%), Erythromycin (54%), Cotrimoxazole (27%) gentamicin (16%), amikacin (12%) piperacillin/tazobactum and linezolide (3% each). The increased recognition of pathogenic potential in CONS and emergence of drug resistance among them demonstrates the need to adopt simple laboratory methods to identify the species and determine the antibiotic resistant patterns. It will help the clinicians in treating the infections caused by CONS.


Keywords: Specimens, Antibiogram, Staphylococcal isolates and Pathogens
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How to cite this article:

Mariraj Jeer, Jyoti R. Hundekar and Krishna, S. 2017. Speciation of Coagulase Negative Staphylococcal Isolates from Clinically Significant Specimens and their Antibiogram.Int.J.Curr.Microbiol.App.Sci. 6(6): 1824-1827. doi: https://doi.org/10.20546/ijcmas.2017.606.212