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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(6): 1740-1749
DOI: https://doi.org/10.20546/ijcmas.2017.606.202


A Study on Fungal Etiological Agents in Suppurative Keratitis
Anna Cherian*, Honey Susan Paul, R. Jyothi and J.T. Ramani Bai
Department of Microbiology, Govt. Medical College, Trivandrum, Kerala, India
*Corresponding author
Abstract:

Fungal keratitis is a major ophthalmic problem. In view of the importance in timely diagnosis and treatment of keratitis which would otherwise lead to serious complications like loss of vision, hypopyon formation, endopthalamitis, etc., this study was intended to bring into light the fungal etiological agents in suppurative keratitis. The specimen was collected from suppurative keratitis cases irrespective of prior antibiotic treatment during the period of one year from March 2012 to February 2013. Those cases with loss of corneal epithelium with signs of inflammation, and stromal infiltration were taken as suppurative keratitis cases. Five or more corneal scrapings are to be taken, based on the condition of the patient. The corneal scrapings are used for 10% KOH mount and Gram staining. Sabouraud’s Dextrose Agar (SDA) is inoculated by touching the medium with the needle. Extreme care is taken to keep the entire process sterile. During the one-year period, out of the 100 patients with suppurative keratitis, there were 56 (82.36%) fungal isolates. Aspergillus spp. was the commonest, constituting 22 (39.29%) of the total fungal isolates. Fusarium is the second most common in this study 9(16.7%). Although corneal ulceration is a very rare cause of mortality, it is one of the important causes of morbidity and disability in South Kerala, especially among lower socio-economic groups. The present study gives an insight about the fungal aetiological agents of suppurative keratitis in this part of Kerala. The results of this study are similar to that in other parts of India. These findings have important public health implications for the treatment and prevention of corneal ulceration in the developing world.


Keywords: Suppurative keratitis, Corneal scrapings, SDA.
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How to cite this article:

Anna Cherian, Honey Susan Paul, R. Jyothi and Ramani Bai, J.T. 2017. A study on Fungal Etiological Agents in Suppurative Keratitis.Int.J.Curr.Microbiol.App.Sci. 6(6): 1740-1749. doi: https://doi.org/10.20546/ijcmas.2017.606.202