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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(6): 1691-1700
DOI: https://doi.org/10.20546/ijcmas.2017.606.197


Isolation and Characterization of N2 Fixing Anaerobic Bacteria from Paddy Ecosystem
Daphy Meurial, C.K. Kumar and U. Sivakumar
Department of Agricultural Microbiology, Tamil Nadu Agricultural University, Coimbatore, India
*Corresponding author
Abstract:

Rice cultivation is taken in flooded soil condition. This creates an anaerobic environment and harbours different group of anaerobes. The continued submergence of soil provides a reducing environment and effects a build-up of anaerobic ecosystem in which two unique processes namely methane emission and reductive transformation of nitrogen occur. But the role played by specific group of anaerobes with respect to nitrogen transformation is still obscure. A study was undertaken to assess the different groups of anaerobes in nitrogen transformation in flooded rice soils. The nitrogen fixing obligate and facultative anaerobes were isolated and characterised from paddy ecosystem. The population of culturable anaerobic bacteria in flooded rice soils was enumerated by the anaerobic roll tube method using Hungates agar medium. Higher AR activity was noticed for the screened obligate and facultative anaerobic bacteria and exhibited maximum activity on 132nd hour (19.81 n moles of C2H4 mg-1 protein h-1. In the present investigation, the occurrences of anaerobic nitrogen fixer in the paddy ecosystem and their role in nitrogen fixation have been examined.


Keywords: Anaerobes, Paddy ecosystem, Diazotrophs, Nitrogen fixation, Acetylene reduction assay.
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How to cite this article:

Daphy Meurial, C.K. Kumar and Sivakumar, U. 2017. Isolation and Characterization of N2 Fixing Anaerobic Bacteria from Paddy Ecosystem.Int.J.Curr.Microbiol.App.Sci. 6(6): 1691-1700. doi: https://doi.org/10.20546/ijcmas.2017.606.197