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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(6): 1609-1618
DOI: https://doi.org/10.20546/ijcmas.2017.606.189


Characterization of Mungbean (Vigna radiata L. Wilczek) Varieties using Morphological and Molecular Descriptors
Rupinder Kaur1*, A.K. Toor1, Geeta Bassi1 and T.S. Bains2
1Seed Technology Centre; 2Pulses Section Department of Plant Breeding and Genetics,Punjab Agricultural University, Ludhiana, Punjab141004
*Corresponding author
Abstract:

Five mungbean varieties released by Punjab Agricultural University (PAU911, SML668, ML818, ML613 and SML832) were characterized using morphological and molecular markers. Phenotypically these varieties showed variation for growth habit, leaf and flower characters, pod colour, position and length, plant height, seed coat lusture and seed size during different growth stages of the crop. Plant morphology characters being polygenic in nature are liable to be influenced by the environment. Hence there is a need to use alternate descriptors which are rapid, accurate and less affected by environment. Therefore, in the present study, simple sequence repeats (SSR) markers were also used for determining genetic diversity analysis and supplementing morphological data. Five mungbean varieties were analysed using 44 SSR molecular markers, covering the whole genome of greengram. Out of 44 primers screened primers RG6, RG7, RG8, RG11, RG14, RG15 and MBM00101exhibited distinct banding pattern. Markers RG6 and RG7 differentiate the variety ML818 and SML832. Marker RG11 distinguished ML818 and ML613. Similarly, primers RG8, RG11, RG14 and RG15 showed distinctive banding pattern among the SML832 and ML613. Primer MBM001101 indicated polymorphism among PAU 911 and SML668. The UPGMA dendrogram based on similarity co-efficient showed maximum genetic difference between ML613 and ML818 and maximum similarity between ML818 and SML668.


Keywords: Genetic diversity, greengram, morphological markers,SSR markers.
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How to cite this article:

Rupinder Kaur, A.K. Toor, Geeta Bassi and Bains, T.S. 2017. Characterization of Mungbean (Vigna radiata L. Wilczek) Varieties using Morphological and Molecular Descriptors.Int.J.Curr.Microbiol.App.Sci. 6(6): 1609-1618. doi: https://doi.org/10.20546/ijcmas.2017.606.189