Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(6): 2589-2593
DOI: https://doi.org/10.20546/ijcmas.2017.606.309


Molecular Detection and Pathology of Necrotic Enteritis Case from a Guinea Fowl
S. Rathnapraba1*, Kannabhiran1, P. Kanagaraju2, K. Vijayarani1 and S. Saranya1
1Department of Animal Biotechnology, 2Department of Poultry Science, Madras Veterinary College, Tamilnadu Veterinary and Animal Sciences University, Chennai-07, India
*Corresponding author
Abstract:

Necrotic enteritis is an important bacterial disease of poultry caused by type A and type C of the enteric gram positive spore forming organism Clostridium perfringens. Necrotic enteritis suspected case was presented for necropsy from a dead guinea fowl. Gross examination showed ulcerative lesions in the intestine. Microscopically, necrosis and degeneration of the intestinal villi and mononuclear cell infiltration was noticed. Liver histopathology revealed that the mononuclear cells infiltration and perivascular cuffing in blood vessels. The causative organism was isolated from the intestinal contents under anaerobic condition. The collected sample was processed and growth was achieved on tryptose – sulphite – cycloserine (TSC) agar, blood agar, clostridial agar, perfringens agar. Typical Clostridium perfringens colonies were identified by grams staining and further confirmed by biochemical characterization. For molecular confirmation genomic DNA was isolated from C. perfringens and amplified using 16s rRNA universal primer. Multiplex PCR (mPCR) was carried out for toxin genotyping and the isolate was found positive for α toxin (cpa), a characteristic feature of C. perfringens type A. The present report emphasizes the molecular characterization and toxin genotyping as a rapid tool for detection of C. perfringens from suspected necrotic enteritis cases in poultry.


Keywords: Rathnapraba, S., Kannabhiran, P. Kanagaraju, K. Vijayarani and Saranya, S. 2017. Molecular Detection and Pathology of Necrotic Enteritis Case from a Guinea Fowl.
Download this article as Download

How to cite this article:

Guinea fowl, mPCR, Necrotic enteritis, Toxin genotypingInt.J.Curr.Microbiol.App.Sci. 6(6): 2589-2593. doi: https://doi.org/10.20546/ijcmas.2017.606.309