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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(6): 2573-2581
DOI: https://doi.org/10.20546/ijcmas.2017.606.306


Molecular Characterization of Fenugreek (Trigonella foenum-graecum L.) Genotypes Using Rapd Markers
N.C. Mamatha*, S.K. Tehlan, M. Srikanth, M.K. Shivaprasad and P. Karthik Reddy
Department of Vegetable Science, CCS Haryana Agricultural University, Hisar-125 004, India
*Corresponding author
Abstract:

The study was conducted to reveal the genetic diversity among 48 fenugreek genotypes using thirty Random Amplified Polymorphic DNA (RAPD) markers among which, nineteen primers gave good amplification and revealed a total of 119 polymorphic bands with an average of 6.26 bands per primer. The percentage of polymorphism ranged from 50.00 to 91.66 per cent with an average of 79.21 % polymorphism per primer. Primer OPP-8 revealed the highest polymorphism (91.66%), whereas the primer OPC-2 exhibited the lowest polymorphism (50.00%). The polymorphic information content (PIC) value ranged from 0.66 to 0.90. Highest PIC value was obtained in primers OPP-8 followed by OPP-9, OPH-5, OPF-13, OPJ-18, OPB-5, OPC-17, OPAB-2, OPH-4 and OPN-2 and the lowest PIC value was obtained in OPC-19 followed by OPC-11 and OPG-11.UPGMA dendrogram presented into 10 clusters at 0.75 similarity coefficient. The similarity coefficient ranging from 0.59 to 1.00. Principal component analysis confirmed the results of UPGMA. The genotypes which are falling in different groups indicate the presence of genetic diversity between the members of different clusters. Crossing can be made between the members of different clusters for further crop improvement, as they are genetically diverse.


Keywords: Fenugreek, diversity, RAPD, UPGMA, PIC.
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How to cite this article:

Mamatha, N.C., S.K. Tehlan, M. Srikanth, M.K. Shivaprasad and Karthik Reddy, P. 2017. Molecular Characterization of Fenugreek (Trigonellafoenum-graecum L.) Genotypes Using Rapd Markers.Int.J.Curr.Microbiol.App.Sci. 6(6): 2573-2581. doi: https://doi.org/10.20546/ijcmas.2017.606.306