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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(6): 2565-2572
DOI: https://doi.org/10.20546/ijcmas.2017.606.305


Asessment of Genetic Diversity among Fenugreek (Trigonella foenum-graecum L.) Genotypes using ISSR Markers
N.C. Mamatha*, S.K. Tehlan, M. Srikanth, T. Ravikumar, Shikha Yashveer and Mukesh Kumar
Department of Vegetable Science, CCS Haryana Agricultural University, Hisar-125004, India
*Corresponding author
Abstract:

Fenugreek [Trigonella foenum-graecum L.] is an annual herb of Leguminaceae family and is largely cultivated in warm temperate and tropical regions. The presence of variability in breeding population is prior need for crop improvement. This calls for an evaluation of genotypes to study the variability. The study was conducted to reveal the genetic diversity among 48 fenugreek genotypes which were collected from different geographical regions of India using Inter Simple Sequence Repeat markers. Totally twenty ISSR primers were screened among which, ten primers gave good amplification and revealed a total of 48 polymorphic bands with an average of 4.8 bands per primer. The percentage of polymorphism ranged from 75.00 % (UBC-842) to 100 % (UBC-851). Highest Polymorphic Information Content (PIC) value was obtained in primers UBC-842 and UBC-860 among ten primers these proved best to assess the genetic diversity in fenugreek. UPGMA dendrogram presented into 10 clusters at 0.75 similarity coefficient. The similarity coefficient ranging from 0.59 to 1.00. Principal component analysis confirmed the results of UPGMA. The genotypes from one region were grouped together in some cases, while they were placed in different clusters in certain cases. The measures of relative genetic distances among varieties of fenugreek did not completely correlate with the geographical distances of their development. And the genotypes which are falling in different groups indicate the presence of genetic diversity between the members of different clusters. Crossing can be made between the members of different clusters for further crop improvement, as they are genetically diverse.


Keywords: Fenugreek, Diversity, ISSR, UPGMA, PIC
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How to cite this article:

Mamatha, N.C., S.K. Tehlan, M. Srikanth, T. Ravikumar, Shikha Yashveer and Mukesh Kumar. 2017. Asessment of Genetic Diversity among Fenugreek (Trigonella foenum-graecum L.) Genotypes using ISSR Markers.
Int.J.Curr.Microbiol.App.Sci. 6(6): 2565-2572. doi: https://doi.org/10.20546/ijcmas.2017.606.305