Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(6): 1347-1353
DOI: https://doi.org/10.20546/ijcmas.2017.606.158


Evaluation of Bio Control Efficacy of Pseudomonas fluorescens AS15 against Banded Leaf and Sheath Blight Pathogen (Rhizoctonia solani) in different Carbon and Nitrogen Sources
Anjul Rana* and Manvika Sahgal
Department of Microbiology, G.B. Pant University of Agriculture and Technology,
Pantnagar, 263145, Uttarakhand, India
*Corresponding author
Abstract:

Banded leaf and sheath blight disease causes major yield loss of maize. Bio control agents control the disease without any harmful effect. Pseudomonas fluorescence is a potential bio control agent due to production lytic enzymes and various secondary metabolites such as DAPG, Phenazine, pyoluteorin, pyrollnitrin. In dual plate assay P. fluorescens AS15 culture and it supernatant inhibit the growth of Rhizoctonia solani with 35.5% and 86.6% percent inhibition respectively. The λ max for DAPG was detected in all carbon and nitrogen sources except tween 20 and Pyoluteorin λ max was also detected in all carbon and nitrogen sources except arabinose and tween 20. Whereas λ max for pyrrolnitrin was detected in the presence of serine, arginine, L-phenylalanine, threonine, arabinose, ducitol, sucrose, glucose and galactose. The best sources for the growth of P. fluorescens AS15 were sucrose and glucose. Good percent inhibition of P. fluorescens AS15 against Rhizoctonia solani was observed in the presence of inulin, dulcitol, glucose, galactose, serine and sucrose. In present study we conclude that glucose is the best carbon source for the growth, production of antibiotic and antagonistic behaviour of P. fluorescens AS15.


Keywords: Banded leaf and sheath blight, Pseudomonas fluorescens,Bio control, Rhizoctonia solani, Maize, Antibiotics.
Download this article as Download

How to cite this article:

Anjul Rana and Manvika Sahgal. 2017. Evaluation of Biocontrol Efficacy of Pseudomonas fluorescens AS15 against Banded Leaf and Sheath Blight Pathogen (Rhizoctonia solani) in Different Carbon and Nitrogen Sources.Int.J.Curr.Microbiol.App.Sci. 6(6): 1347-1353. doi: https://doi.org/10.20546/ijcmas.2017.606.158